Renoprotective Effect of Total Glucosides of Paeony (TGP) and Its Mechanism in Experimental Diabetes
| Autor: | Yong-Gui Wu, Jing Dong, Kejun Ren, Xiang-Ming Qi, Ji-Jia Shen, Shanyan Lin, Chao Liang, Liang Yuan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Kidney Paeonia Plant Roots Collagen Type I Diabetes Mellitus Experimental Excretion Nephrin Transforming Growth Factor beta1 Western blot Glucosides Neurotrophin 3 Internal medicine medicine Animals Rats Wistar Triglycerides Pharmacology medicine.diagnostic_test biology Dose-Response Relationship Drug business.industry Tumor Necrosis Factor-alpha Body Weight lcsh:RM1-950 Transcription Factor RelA Interleukin Membrane Proteins Intercellular adhesion molecule Intercellular Adhesion Molecule-1 Rats Endocrinology medicine.anatomical_structure Cholesterol lcsh:Therapeutics. Pharmacology Creatinine biology.protein Molecular Medicine Tumor necrosis factor alpha business Transforming growth factor Interleukin-1 Phytotherapy |
| Zdroj: | Journal of Pharmacological Sciences, Vol 109, Iss 1, Pp 78-87 (2009) |
| ISSN: | 1347-8613 |
| Popis: | Total glucosides of paeony (TGP), extracted from the root of Paeonia lactiflora pall, has been shown to have ant-inflammatory and antioxidative actions. The aims of this study were to elucidate the renoprotective effect of TGP and its mechanism in experimental diabetes. Streptozotocin-induced diabetic rats were treated with TGP for 8 weeks. Treatment with TGP at 50, 100, and 200 mg/kg significantly lowered 24-h urinary albumin excretion rate in diabetic rats. TGP treatment in all doses markedly attenuated glomerular volume, and treatment with TGP at 100 and 200 mg/kg markedly reduced indices for tubulointerstitial injury in diabetic rats. Western blot analysis showed that the expressions of 1α (IV) collagen, intercellular adhesion molecule (ICAM)-1, interleukin (IL)-1, tumor necrosis factor (TNF)-α, NF-κB p65, and 3-nitrotyrosine (3-NT) protein were increased in the kidneys of diabetic rats; the increases in these proteins were all dose-dependently and significantly inhibited by TGP treatment. The expression of nephrin protein was significantly reduced in the kidneys from diabetic rats and markedly increased by TGP treatment. The expression of transforming growth factor (TGF)-β1 protein in the kidney was also significantly increased in diabetic rats, which was significantly inhibited by treatment with TGP at all doses. Our data suggest that TGP treatment ameliorates early renal injury via the inhibition of expression of ICAM-1, IL-1, TNF-α, and 3-NT in the kidneys of diabetic rats. Keywords:: diabetic nephropathy, total glucosides of paeony (TGP), intercellular adhesion molecule (ICAM)-1, interleukin (IL)-1, tumor necrosis factor (TNF)-α |
| Databáze: | OpenAIRE |
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