Pdgfrβ+ Mural Preadipocytes Contribute to Adipocyte Hyperplasia Induced by High-Fat-Diet Feeding and Prolonged Cold Exposure in Adult Mice
| Autor: | Stephen B. Spurgin, Margaret Y. Wang, Thomas S. Morley, Lavanya Vishvanath, Qiong A. Wang, Rana K. Gupta, Mengle Shao, Christine M. Kusminski, Chelsea Hepler, Karen A. MacPherson |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Aging Physiology Adipose Tissue White Adipose tissue Inflammation Cell Count Mice Transgenic White adipose tissue Biology Diet High-Fat Mural cell Article Receptor Platelet-Derived Growth Factor beta 03 medical and health sciences chemistry.chemical_compound Adipose Tissue Brown Internal medicine Adipocyte medicine Adipocytes Animals Cell Lineage Molecular Biology Adipogenesis Hyperplasia Stem Cells Cell Membrane Cell Biology Feeding Behavior medicine.disease Cold Temperature DNA-Binding Proteins Mice Inbred C57BL 030104 developmental biology Endocrinology chemistry Female medicine.symptom Stem cell Biomarkers Transcription Factors |
| Zdroj: | Cell metabolism. 23(2) |
| ISSN: | 1932-7420 |
| Popis: | The expansion of white adipose tissue (WAT) in obesity involves de novo differentiation of new adipocytes; however, the cellular origin of these cells remains unclear. Here, we utilize Zfp423(GFP) reporter mice to characterize adipose mural (Pdgfrβ(+)) cells with varying levels of the preadipocyte commitment factor Zfp423. We find that adipose tissue contains distinct mural populations, with levels of Zfp423 distinguishing adipogenic from inflammatory-like mural cells. Using our "MuralChaser" lineage tracking system, we uncover adipose perivascular cells as developmental precursors of adipocytes formed in obesity, with adipogenesis and precursor abundance regulated in a depot-dependent manner. Interestingly, Pdgfrβ(+) cells do not significantly contribute to the initial cold-induced recruitment of beige adipocytes in WAT; it is only after prolonged cold exposure that these cells differentiate into beige adipocytes. These results provide genetic evidence for a mural cell origin of white adipocytes in obesity and suggest that beige adipogenesis may originate from multiple sources. |
| Databáze: | OpenAIRE |
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