Microbiological Investigations of ReNu Plastic Bottles and the 2004 to 2006 ReNu With MoistureLoc-Related Worldwide Fusarium Keratitis Event
| Autor: | Harry J. Khamis, Ronald E. Warwar, John D. Bullock, B. Laurel Elder |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Fusarium business.product_category Hot Temperature Contact Lenses Polymers Drug Storage 030106 microbiology Biguanides 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Bottle Food science Alexidine Drug Packaging biology Chemistry business.industry food and beverages biology.organism_classification Biotechnology Anti-Bacterial Agents Contact lens Ophthalmology 030221 ophthalmology & optometry Contact Lens Solutions Glass business Plastics |
| Zdroj: | Eyecontact lens. 42(3) |
| ISSN: | 1542-233X |
| Popis: | Purposes The purposes of this study were to determine whether the contact lens solution RevitaLens Ocutec (containing the antimicrobial agents alexidine and polyquaternium-1) would inhibit Fusarium organisms when heated in ReNu plastic bottles; whether alexidine would inhibit Fusarium organisms when heated in non-ReNu plastic bottles; and whether an alexidine-neutralizing compound leaches from heated ReNu bottles. Methods RevitaLens and an alexidine solution (0.00045%), previously stored in ReNu bottles at room temperature (RT) and 56°C, were incubated with 7 different Fusarium organisms. The alexidine solution was similarly stored in seven non-ReNu plastic bottles and incubated with these same organisms. To determine if an alexidine-neutralizing compound might be leaching from heated ReNu bottles, phosphate-buffered saline (PBS) was incubated at RT and 56°C in ReNu bottles, combined with alexidine, and then tested for anti-Fusarium capability. Results After being heated in ReNu bottles, RevitaLens retained its anti-Fusarium capability, whereas the alexidine solution did not. The alexidine solution heated in seven non-ReNu plastic bottles retained its anti-Fusarium capability. The alexidine solution retained its anti-Fusarium capability when incubated with a PBS solution that had been heated in ReNu bottles, indicating, microbiologically, that an alexidine-neutralizing compound did not leach from the heated ReNu bottle. Conclusions Alexidine uniquely fails to inhibit Fusarium organisms when heated in a plastic ReNu bottle, but not in seven other plastic bottles, whereas the anti-Fusarium capability of RevitaLens (containing the antimicrobial agents alexidine and polyquaternium-1) is unaffected by heating in a ReNu bottle. There does not seem to be an alexidine-neutralizing compound leaching from heated ReNu bottles. An interaction between alexidine and its heated ReNu bottle may have been a critical factor in the worldwide ReNu with MoistureLoc-related Fusarium keratitis event of 2004 to 2006. |
| Databáze: | OpenAIRE |
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