CD7‐negative T cells represent a separate differentiation pathway in a subset of post‐thymic helper T cells
| Autor: | U. Reinhold, J Sesterhenn, Lide Liu, Hinrich Abken |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Immunology
Antigens CD7 Lymphocyte Activation Immunophenotyping Interleukin 21 T-Lymphocyte Subsets hemic and lymphatic diseases Humans Immunology and Allergy Cytotoxic T cell RNA Messenger IL-2 receptor Antigen-presenting cell Interleukin 3 CD40 biology CD28 Cell Differentiation T-Lymphocytes Helper-Inducer Blotting Northern Natural killer T cell Molecular biology Gene Expression Regulation biology.protein Research Article |
| Zdroj: | Immunology. 89:391-396 |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | The absence of CD7 protein and the corresponding mRNA is a stable feature in a subset of normal circulating CD4+ memory T cells. It is still unresolved whether the CD7- subset represents a specific T-cell lineage. Here we show that repeated stimulation of highly purified CD4+ CD45RA+ CD45RO- naive T cells in vitro leads to the development of a distinct memory subset that is defined by the expression versus non-expression of the CD7 antigen. Comparing different T-cell activation pathways (TCR/CD3, CD2), we observed that alternative signals were critically involved in the development of CD4+ CD7- T cells. Peak mean numbers of CD7- memory cells occurred after 3-5 cycles of restimulation in vitro. Naive T cells that had undergone repeated stimulations were harvested and sorted into CD7+ and CD7- subsets. The vast majority (> 97%) of CD7+ T cells retained their expression, whereas the CD7- population did not re-express the antigen during further propagation of separated T-cell subsets. In CD7- cells no CD7 mRNA was monitored, indicating transcriptional regulation of CD7 expression. Certain differentiation-related antigens, including the cutaneous lymphocyte antigen CLA, were preferentially expressed on CD7- T cells. We suggest that absence of CD7 expression in a subset of CD4+ memory cells reflects a separate and stable differentiation state occurring late in the immune response. These T cells may represent the physiological counterpart of malignant T cells in certain forms of cutaneous T-cell lymphoma. |
| Databáze: | OpenAIRE |
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