A specific PCR for the identification of Mycoplasma capricolum subsp. capripneumoniae, the causative agent of contagious caprine pleuropneumonia (CCPP)
| Autor: | François Thiaucourt, Armelle Peyraud, Lucia Manso-Silvan, Sophie Lorenzon, Salah Woubit |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Hydrolases Molecular Sequence Data L73 - Maladies des animaux medicine.disease_cause Polymerase Chain Reaction Microbiology Mycoplasma capricolum law.invention Contagious caprine pleuropneumonia Mycoplasma law RNA Ribosomal 16S medicine Animals Surveillance épidémiologique Pleuropneumonia Contagious Arginine deiminase pathway Polymerase chain reaction Goat Diseases Base Sequence General Veterinary biology Goats Mycoplasma mycoides General Medicine biology.organism_classification medicine.disease Virology PCR Pleuropneumonia Mollicutes Mycoplasmose |
| Zdroj: | Veterinary Microbiology |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2004.08.006 |
| Popis: | Contagious caprine pleuropneumonia is a severe infectious disease of goats in Africa and the Middle East. It is caused by a fastidious mycoplasma, Mycoplasma capricolum subsp. capripneumoniae, a member of the "M. mycoides cluster". Members of this cluster share genomic and antigenic features, which result in common biochemical and serological properties, complicating species identification. Two species of this cluster, M. mycoides subsp. capri and M. mycoides subsp. mycoides large colony biotype, are very often isolated from clinical cases resembling contagious caprine pleuropneumonia. Furthermore, in the laboratory, M. capricolum subsp. capripneumoniae can be easily confused with the closely related capricolum subspecies. Considering these constraints and the scarcity of available methods for identification, a specific polymerase chain reaction was developed. A DNA fragment of 7109 bp containing genes coding for the arginine deiminase pathway (ADI) was chosen as target sequence for the selection of a specific primer pair. The full ADI operon from M. capricolum subsp. capripneumoniae strain GL100 was sequenced. Polymorphism within this locus was analyzed by comparison with the sequence from the closely related IPX strain (M. capricolum subsp. capricolum). It varied from 0.6% to 3.5%. The highest divergence was found in a region coding for arcD. Therefore, this gene was chosen as target for the specific amplification of a 316 bp-long DNA fragment. The specificity of this PCR was validated on 14 M. capricolum subsp. capripneumoniae strains and 27 heterologous strains belonging to the "M. mycoides cluster" and M. putrefaciens. This new PCR will be a valuable tool for the surveillance of contagious caprine pleuropneumonia. |
| Databáze: | OpenAIRE |
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