Targeting the Bacterial Cytoskeleton of the Burkholderia cepacia Complex for Antimicrobial Development: A Cautionary Tale

Autor: Sonya Carnell, Marcella Facchini, Anjam C M Khan, Anthony De Soyza, Alba Silipo, Alessandra Bragonzi, John D. Perry, Waldemar Vollmer, Annette C. Vergunst, Lee A. Borthwick, Jacob Biboy, Daniela Vollmer
Přispěvatelé: Institute of Cellular Medicine [Newcastle], Newcastle University [Newcastle], Institute for Cell and Molecular Biosciences, IRCCS Ospedale San Raffaele [Milan, Italy], Università degli studi di Napoli Federico II, Virulence bactérienne et maladies infectieuses (VBMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Carnell, Sonya C, Perry, John D, Borthwick, Lee, Vollmer, Daniela, Biboy, Jacob, Facchini, Marcella, Bragonzi, Alessandra, Silipo, Alba, Vergunst, Annette C, Vollmer, Waldemar, Khan, Anjam C M, De Soyza, Anthony
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: International Journal of Molecular Sciences
Volume 19
Issue 6
International Journal of Molecular Sciences, MDPI, 2018, 19 (6), pp.1604. ⟨10.3390/ijms19061604⟩
International Journal of Molecular Sciences, Vol 19, Iss 6, p 1604 (2018)
ISSN: 1422-0067
1661-6596
DOI: 10.3390/ijms19061604
Popis: Burkholderia cepacia complex (BCC) bacteria are a group of opportunistic pathogens that cause severe lung infections in cystic fibrosis (CF). Treatment of BCC infections is difficult, due to the inherent and acquired multidrug resistance of BCC. There is a pressing need to find new bacterial targets for antimicrobials. Here, we demonstrate that the novel compound Q22, which is related to the bacterial cytoskeleton destabilising compound A22, can reduce the growth rate and inhibit growth of BCC bacteria. We further analysed the phenotypic effects of Q22 treatment on BCC virulence traits, to assess its feasibility as an antimicrobial. BCC bacteria were grown in the presence of Q22 with a broad phenotypic analysis, including resistance to H2O2-induced oxidative stress, changes in the inflammatory potential of cell surface components, and in-vivo drug toxicity studies. The influence of the Q22 treatment on inflammatory potential was measured by monitoring the cytokine responses of BCC whole cell lysates, purified lipopolysaccharide, and purified peptidoglycan extracted from bacterial cultures grown in the presence or absence of Q22 in differentiated THP-1 cells. BCC bacteria grown in the presence of Q22 displayed varying levels of resistance to H2O2-induced oxidative stress, with some strains showing increased resistance after treatment. There was strain-to-strain variation in the pro-inflammatory ability of bacterial lysates to elicit TNF&alpha
and IL-1&beta
from human myeloid cells. Despite minimal toxicity previously shown in vitro with primary CF cell lines, in-vivo studies demonstrated Q22 toxicity in both zebrafish and mouse infection models. In summary, destabilisation of the bacterial cytoskeleton in BCC, using compounds such as Q22, led to increased virulence-related traits in vitro. These changes appear to vary depending on strain and BCC species. Future development of antimicrobials targeting the BCC bacterial cytoskeleton may be hampered if such effects translate into the in-vivo environment of the CF infection.
Databáze: OpenAIRE
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