Lentiviral-Mediated Silencing of Farnesyl Pyrophosphate Synthase through RNA Interference in Mice
| Autor: | Jian Yang, Chen-Ze Zhao, Fei Chen, Jie Han, Shen-Jiang Hu, Bin Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genetically modified mouse
Article Subject Transgene Farnesyl pyrophosphate lcsh:Medicine Mice Transgenic Biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Mice RNA interference Gene silencing Animals Myocytes Cardiac Gene Cells Cultured General Immunology and Microbiology lcsh:R Lentivirus Geranyltranstransferase General Medicine Molecular biology Mice Inbred C57BL chemistry RNA Interference Mevalonate pathway Research Article Plasmids |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2015 (2015) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Farnesyl pyrophosphate synthase (FPPS) plays a vital role in the mevalonate pathway and has been shown to be involved in hypertrophy and cardiovascular diseases. Lentivirus-mediated RNA interference (RNAi) to knock down a gene of interest has become a promising new tool for the establishment of transgenic animals. The interfering fragment, named pLVT202, was chosen from cardiomyocytes tested in vitro and was microinjected into the perivitelline space of zygotes from C57BL/6J mice via a lentivirus vehicle; 20 were identified as carrying copies of the transgene using the polymerase chain reaction (PCR). Real-time PCR and western blotting analysis showed that FPPS was downregulated in multiple tissues in the transgenic mice. The transgenic mouse model provides a novel means of studying the gene function of FPPS. |
| Databáze: | OpenAIRE |
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