Ceratitis capitata transformer-2 gene is required to establish and maintain the autoregulation of Cctra, the master gene for female sex determination
| Autor: | Giuseppe Saccone, Peter W. Atkinson, Mark Robertson, Marco Salvemini, Lino C. Polito, Benjamin Aronson |
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| Přispěvatelé: | Salvemini, Marco, Robertson, M, Aronson, B, Atkinson, P, Polito, Catello, Saccone, Giuseppe |
| Rok vydání: | 2009 |
| Předmět: |
Male
Embryology Transcription Genetic Female sex determination Genome Insect Molecular Sequence Data Doublesex sex determination Nerve Tissue Proteins splicing Sexual Behavior Animal Splicing factor evolution Animals Amino Acid Sequence Cloning Molecular Conserved Sequence Genetics Sex Chromosomes biology autoregulation Alternative splicing sexual behaviour Gene Expression Regulation Developmental Nuclear Proteins Ceratitis capitata Sex Determination Processes Sex reversal biology.organism_classification DNA-Binding Proteins Alternative Splicing Phenotype RNA splicing Female RNA Interference fruitless Drosophila melanogaster Sequence Alignment Developmental Biology |
| Zdroj: | The International Journal of Developmental Biology. 53:109-120 |
| ISSN: | 0214-6282 |
| DOI: | 10.1387/ijdb.082681ms |
| Popis: | In Drosophila melanogaster, transformer-2 (TRA-2) which is a non-sex-specific auxiliary splicing factor, is required to promote female sexual differentiation by interaction with the female-specific TRA. The two proteins positively regulate the splicing of both doublesex (dsx) and fruitless (fru) pre-mRNAs, which in turn regulate phenotypic and behavioural sexual dimorphism. In the Mediterranean fruitfly Ceratitis capitata, the female-specific CcTRA is similarly required not only for Ccdsx splicing, but also to exert a novel autoregulatory function that consists of promoting female-specific splicing of Cctra pre-mRNA. This study reports the isolation and functional analysis of the C. capitata homologue of the Drosophila transformer-2 gene (Cctra-2). Transient RNAi against Cctra-2 during embryonic development causes the full sex reversal of XX flies in adult fertile pseudo-males, as well as changes in the splicing pattern of Cctra, Ccdsx and Ccfruitless (Ccfru). We propose that: 1) Cctra-2, as in Drosophila, is necessary for promoting Ccdsx and putative Ccfru pre-mRNA female-specific splicing and that 2) unlike in Drosophila, Cctra-2 appears to be necessary for establishing female sex determination in early XX embryos and for maintaining the positive feedback regulation of Cctra during development. |
| Databáze: | OpenAIRE |
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