Enhanced percutaneous delivery of recombinant human epidermal growth factor employing nano-liposome system

Autor:	Min-Sun Park, Kyeung-Hwa Chun, Chang-Woo Suh, Sun-Mi Hong, Won-Chul Kim, Jo-Eun Seo, Sang-kil Lee, Sang-Ok Jeon, Chae-Ha Yoon, Hee-Jin Hwang, Dong Ho Oh, Kyunghyun Min, Min-Ju Kim
Rok vydání:	2012
Předmět:	Male Microscope Materials science Analytical chemistry Pharmaceutical Science Bioengineering Administration Cutaneous Permeability law.invention Diffusion Rats Sprague-Dawley Drug Delivery Systems Colloid and Surface Chemistry Microscopy Electron Transmission law Zeta potential Animals Humans Nanotechnology NLS Particle Size Physical and Theoretical Chemistry Chromatography High Pressure Liquid Skin Liposome Microscopy Confocal Epidermal Growth Factor Vesicle Organic Chemistry Permeation Rats Microscopy Fluorescence Transmission electron microscopy Liposomes Biophysics Nanoparticles Particle size
Zdroj:	Journal of Microencapsulation. 29:234-241
ISSN:	1464-5246 0265-2048
DOI:	10.3109/02652048.2011.646327
Popis:	We encapsulated recombinant human epidermal growth factor (rhEGF) into nano-liposomes (NLs) system for topical delivery. The rhEGF-loaded NLs were prepared using a high pressure homogenization method. Morphology and overall particle distribution of NLs were investigated using transmission electron microscopy (TEM) and high resolution microscope (CytoViva™). Particle size, zeta (ζ) potential and encapsulation efficiency were measured and the percutaneous delivery of NLs was evaluated using Franz diffusion cells and immunofluorescence confocal laser scanning microscopy (CLSM). The mean particle size, zeta potential and encapsulation efficiency of the NLs were 155.57 ± 2.59 nm, -57.92 ± 4.35 mV and 9.00 ± 0.39%, respectively. TEM and microscopic analysis showed spherical, very even-sized vesicles approximately 150 nm. The skin permeation and localization of rhEGF were enhanced by NLs. CLSM image analysis provided that the NLs enhanced the permeation and localization of rhEGF in rat skin by facilitating entry through pores of skin.
Databáze:	OpenAIRE
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