Differential diagnosis of prostate cancer and benign prostate hyperplasia using two-dimensional electrophoresis
| Autor: | Jean-Philippe Charrier, Michel Jolivet, Jacques Passagot, Pascal Dalbon, Sandrine Michel, Carole Tournel, Serge Comby, Colette Jolivet-Reynaud, Denis Chautard |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male PCA3 medicine.medical_specialty Clinical Biochemistry Prostatic Hyperplasia Urology urologic and male genital diseases Biochemistry Analytical Chemistry Diagnosis Differential Prostate cancer Prostate medicine Humans Electrophoresis Gel Two-Dimensional Aged Tumor marker Aged 80 and over business.industry Prostatic Neoplasms Middle Aged Prostate-Specific Antigen Hyperplasia medicine.disease Prostate-specific antigen medicine.anatomical_structure Differential diagnosis business Benign prostate |
| Zdroj: | ELECTROPHORESIS. 22:1861-1866 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/1522-2683(200105)22:9<1861::aid-elps1861>3.0.co;2-6 |
| Popis: | Prostate specific antigen (PSA) is a protease which is characteristic of the prostate. It is widely used as a serum marker for the early diagnosis of prostate cancer (PCa). Nevertheless, for concentrations between 4 and 10 ng/mL, PSA does not enable PCa to be distinguished from benign diseases, such as benign prostate hyperplasia (BPH). In sera, the use of a ratio between free PSA (PSA uncomplexed with protease inhibitor) and total PSA (free PSA and PSA bound to alpha-1 anti-chymotrypsin) enables the "gray zone" to be reduced, but an important proportion of patients are still wrongly classed. Using two-dimensional electrophoresis, we demonstrated using 52 PCa and 40 BPH well-documented clinical cases that BPH sera show a significantly greater percentage of low-molecular-weight free PSA elements (IwPSA) than PCa sera. In our study, the use of a ratio between IwPSA and standard free PSA enables the correct diagnosis of 100% of PCa and 82.5% of BPH cases as against when 73.1% and 42.5% respectively were correctly diagnozed using the total PSA and the free/total PSA ratio. This important finding may be related to differences in the mechanism secreting PSA from the prostate into the bloodstream. We have shown how a tissue marker may be turned into a powerful tumor marker by events probably unrelated to its expression. |
| Databáze: | OpenAIRE |
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