Rapid Evaluation of Platelet Function With T2 Magnetic Resonance
| Autor: | Adam Cuker, Walter Massefski, Holleh D Husseinzadeh, Thomas Jay Lowery, Michele P. Lambert, Douglas B. Cines, Joseph E. Marturano, John W. Weisel, Tatiana Lebedeva, Charles S. Abrams |
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| Rok vydání: | 2016 |
| Předmět: |
Blood Platelets
0301 basic medicine Pathology medicine.medical_specialty Magnetic Resonance Spectroscopy Platelet Aggregation Platelet Function Tests Platelet disorder Blood volume Clot retraction 030204 cardiovascular system & hematology Pharmacology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Humans Medicine Platelet Whole blood In vitro diagnostic devices Aspirin business.industry T2 magnetic resonance Blood clotting Original Articles General Medicine Platelet Activation 3. Good health Adenosine diphosphate 030104 developmental biology Epinephrine chemistry Arachidonic acid Blood Platelet Disorders business Platelet Aggregation Inhibitors medicine.drug |
| Zdroj: | American Journal of Clinical Pathology |
| ISSN: | 1943-7722 0002-9173 |
| Popis: | Objectives: The clinical diagnosis of qualitative platelet disorders (QPDs) based on light transmission aggregometry (LTA) requires significant blood volume, time, and expertise, all of which can be barriers to utilization in some populations and settings. Our objective was to develop a more rapid assay of platelet function by measuring platelet-mediated clot contraction in small volumes (35 µL) of whole blood using T2 magnetic resonance (T2MR). Methods: We established normal ranges for platelet-mediated clot contraction using T2MR, used these ranges to study patients with known platelet dysfunction, and then evaluated agreement between T2MR and LTA with arachidonic acid, adenosine diphosphate, epinephrine, and thrombin receptor activator peptide. Results: Blood from 21 healthy donors was studied. T2MR showed 100% agreement with LTA with each of the four agonists and their cognate inhibitors tested. T2MR successfully detected abnormalities in each of seven patients with known QPDs, with the exception of one patient with a novel mutation leading to Hermansky-Pudlak syndrome. T2MR appeared to detect platelet function at similar or lower platelet counts than LTA. Conclusions: T2MR may provide a clinically useful approach to diagnose QPDs using small volumes of whole blood, while also providing new insight into platelet biology not evident using plasma-based platelet aggregation tests. |
| Databáze: | OpenAIRE |
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