Method for Measuring in Vivo Oxygen Transport Rates in a Bioartificial Organ
| Autor: | Ronald L. Fournier, Zhan Ding, David W. Whalen |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Islets of Langerhans Transplantation
chemistry.chemical_element Models Biological Oxygen Permeability Diabetes Mellitus Experimental Cell membrane Islets of Langerhans Oxygen permeability Dogs In vivo medicine Animals Bioartificial Organ Cell Membrane General Engineering Oxygen transport Membranes Artificial Haplorhini In vitro Cell biology Membrane medicine.anatomical_structure chemistry Artificial Organs |
| Zdroj: | Tissue Engineering. 5:81-89 |
| ISSN: | 1557-8690 1076-3279 |
| DOI: | 10.1089/ten.1999.5.81 |
| Popis: | Oxygen transport is crucial for the proper functioning of a bioartificial organ. In many cases, the immunoisolation membrane used to protect the transplanted cells from the host's immune system can be a significant barrier to oxygen transport. A method is described for measuring the in vitro and in vivo oxygen transport characteristics of a planar immunoisolation membrane. The in vitro oxygen permeability of the membrane was found to equal 9.22 x 10(-4) cm/sec and was essentially the same as the in vivo value of 9.51 x 10(-4) cm/sec. The fact that the in vitro and in vivo membrane permeabilities are identical indicates that any fibrotic tissue adjacent to the immunoisolation membrane did not present a significant resistance to the transport of oxygen. The measured oxygen permeability was also found consistent with the solute permeabilities obtained in a previous study for larger molecules. Based on the oxygen permeability results, theoretical calculations for this particular membrane indicate that about 1,100 islets of Langerhans/cm2 of membrane area can be sustained at high tissue densities and only 660 islets/cm2 can be supported at low tissue densities. |
| Databáze: | OpenAIRE |
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