A Novel Role of Classical Swine Fever Virus Erns Glycoprotein in Counteracting the Newcastle Disease Virus (NDV)-mediated IFN-β Induction
| Autor: | Yan-hua Xia, Chu-Yu Zhang, Zishu Pan, Liu Chen |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Swine
RNase P Green Fluorescent Proteins Newcastle disease virus Transfection Biochemistry Newcastle disease Virus Cell Line Green fluorescent protein Ribonucleases Viral Envelope Proteins Animals Luciferase RNA Messenger Luciferases Promoter Regions Genetic Molecular Biology Glycoproteins chemistry.chemical_classification Messenger RNA biology Reverse Transcriptase Polymerase Chain Reaction Interferon-beta General Medicine biology.organism_classification Virology Molecular biology Kinetics Gene Expression Regulation chemistry Classical Swine Fever Virus Classical swine fever Glycoprotein Signal Transduction |
| Zdroj: | BMB Reports. 40:611-616 |
| ISSN: | 1976-6696 |
| DOI: | 10.5483/bmbrep.2007.40.5.611 |
| Popis: | E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) and has an unusual feature of RNase activity. In the present study, we demonstrate that E(rns) counteracts Newcastle disease virus (NDV)-mediated induction of IFN-beta. For this purpose, E(rns) fused to the enhanced green fluorescent protein (EGFP) was transiently expressed in porcine kidney 15 (PK15) cells. In luciferase activity assay, E(rns)-EGFP was found to prevent IFN-beta promoter-driven luciferase expression and block the induction of IFN-beta promoter mediated by NDV in a dosedependent manner. Through IFN-specific semi-quantitative RT-PCR detection, obvious decrease of IFN-beta mRNA in NDV-infected PK15 cells was observed in the presence of E(rns)-EGFP. In contrast, EGFP alone showed none of this block capacity. In addition, E(rns)-EGFP mutations with RNase inactivation were also found to block NDV-mediated induction of IFN-beta. These evidences establish a novel function for CSFV E(rns) glycoprotein in counteraction of the IFN-beta induction pathway. |
| Databáze: | OpenAIRE |
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