A proposal to rename the hyperthermophilePyrococcus woeseiasPyrococcus furiosussubsp.woesei
| Autor: | Wirojne Kanoksilapatham, Jocelyne DiRuggiero, Dennis L. Maeder, Juan M. Gonzalez, Frank T. Robb |
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| Rok vydání: | 2004 |
| Předmět: |
Geologic Sediments
Pyrococcus Article Subject Physiology Acid Phosphatase Molecular Sequence Data Context (language use) RNA Archaeal DNA Ribosomal Synteny Microbiology Pyrococcus woesei Terminology as Topic DNA Ribosomal Spacer rRNA Operon Insertion sequence Phylogeny Research Articles Ecology Evolution Behavior and Systematics Recombination Genetic Genetics biology Genes rRNA Sequence Analysis DNA biology.organism_classification QR1-502 Hyperthermophile Blotting Southern RNA Ribosomal 23S DNA Archaeal GenBank DNA Transposable Elements Pyrococcus furiosus RRNA Operon Hydrogen |
| Zdroj: | Archaea, Vol 1, Iss 4, Pp 277-283 (2004) |
| ISSN: | 1472-3654 1472-3646 |
| Popis: | Pyrococcusspecies are hyperthermophilic members of the order Thermococcales, with optimal growth temperatures approaching 100 °C. All species grow heterotrophically and produce H2or, in the presence of elemental sulfur (S°), H2S.Pyrococcus woeseiandP. furiosuswere isolated from marine sediments at the same Vulcano Island beach site and share many morphological and physiological characteristics. We report here that the rDNA operons of these strains have identical sequences, including their intergenic spacer regions and part of the 23S rRNA. Both species grow rapidly and produce H2in the presence of 0.1% maltose and 10–100 µM sodium tungstate in S°-free medium. However,P. woeseishows more extensive autolysis thanP. furiosusin the stationary phase.PyrococcusfuriosusandP. woeseishare three closely related families of insertion sequences (ISs). A Southern blot performed with IS probes showed extensive colinearity between the genomes ofP. woeseiandP. furiosus.Cloning and sequencing of ISs that were in different contexts inP. woeseiandP. furiosusrevealed that thenapAgene inP. woeseiis disrupted by a type III IS element, whereas inP. furiosus, this gene is intact. A type I IS element, closely linked to thenapAgene, was observed in the same context in bothP. furiosusandP. woeseigenomes. Our results suggest that the IS elements are implicated in genomic rearrangements and reshuffling in these closely related strains. We propose to renameP. woeseia subspecies ofP. furiosusbased on their identical rDNA operon sequences, many common IS elements that are shared genomic markers, and the observation that allP. woeseinucleotide sequences deposited in GenBank to date are > 99% identical toP. furiosussequences. |
| Databáze: | OpenAIRE |
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