Programming molecular topologies from single-stranded nucleic acids
| Autor: | Wah Chiu, Shuoxing Jiang, Baoquan Ding, Zhaoming Su, Fei Zhang, Yan Liu, Dongran Han, Xiaodong Qi, Hao Yan, Peng Yin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Science
General Physics and Astronomy 02 engineering and technology Microscopy Atomic Force 010402 general chemistry Network topology 01 natural sciences Article General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Chain (algebraic topology) stomatognathic system Nucleic Acids Nucleotide lcsh:Science chemistry.chemical_classification Physics Quantitative Biology::Biomolecules Multidisciplinary Cryoelectron Microscopy RNA food and beverages DNA General Chemistry Polymer 021001 nanoscience & nanotechnology Quantitative Biology::Genomics 0104 chemical sciences Folding (chemistry) surgical procedures operative chemistry Nucleic acid Nucleic Acid Conformation lcsh:Q 0210 nano-technology Biological system |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Molecular knots represent one of the most extraordinary topological structures in biological polymers. Creating highly knotted nanostructures with well-defined and sophisticated geometries and topologies remains challenging. Here, we demonstrate a general strategy to design and construct highly knotted nucleic acid nanostructures, each weaved from a single-stranded DNA or RNA chain by hierarchical folding in a prescribed order. Sets of DNA and RNA knots of two- or three-dimensional shapes have been designed and constructed (ranging from 1700 to 7500 nucleotides), and they exhibit complex topological features, with high crossing numbers (from 9 up to 57). These single-stranded DNA/RNA knots can be replicated and amplified enzymatically in vitro and in vivo. This work establishes a general platform for constructing nucleic acid nanostructures with complex molecular topologies. Designing nucleic acid-based nanostructures with knots remains challenging. Here the authors present a general strategy to design and construct highly knotted 2D and 3D nanostructures from single-stranded DNA or RNA |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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