Wound healing activity and phytochemical screening of purified fractions of Sempervivum tectorum L. leaves on HCT 116

Autor: Francesca Duraturo, Fabio Cattaneo, Melania Parisi, Maria Iorizzi, Rosario Ammendola, Claudia Finamore, Martina Castaldo, Cristiana Zollo, Carmen Festa, Simona De Marino, Franco Zollo
Přispěvatelé: Cattaneo, Fabio, DE MARINO, Simona, Parisi, Melania, Festa, Carmen, Castaldo, Martina, Finamore, Claudia, Duraturo, Francesca, Zollo, Cristiana, Ammendola, Rosario, Zollo, Franco, Iorizzi, Maria
Rok vydání: 2019
Předmět:
Phytochemicals
wound healing activity
Plant Science
Crassulaceae
01 natural sciences
Biochemistry
High-performance liquid chromatography
Sempervivum tectorum L
Analytical Chemistry
chemistry.chemical_compound
Transactivation
NMR spectroscopy
sedoheptulose
Western blot
Drug Discovery
medicine
Humans
Chromatography
High Pressure Liquid
Cell Proliferation
Wound Healing
biology
medicine.diagnostic_test
Cell growth
2-C-Methyl-D-erythritol
2‐C‐Methyl‐D‐erythritol
NMR spectroscopy
sedoheptulose
Sempervivum tectorumL.
woundhealing activity
Plant Extracts
Spectrum Analysis
010401 analytical chemistry
Cell migration
General Medicine
biology.organism_classification
HCT116 Cells
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Sedoheptulose
Complementary and alternative medicine
chemistry
Molecular Medicine
Wound healing
Food Science
Signal Transduction
Zdroj: Phytochemical analysis : PCA. 30(5)
ISSN: 1099-1565
Popis: Introduction Sempervivum tectorum L. (Crassulaceae), is a succulent perennial plant widespread in Mediterranean countries and commonly used in traditional medicine for ear inflammation, ulcers and skin rashes as a refrigerant and astringent. Objective To demonstrate the therapeutic effects of the plant, various fractions were purified and characterised. The potential wound healing activity, proliferation rate and intracellular signalling cascades were investigated by using human epithelial colorectal carcinoma (HCT 116) cells. Methodology An extraction method without organic solvents was applied for the first time. The purification was carried out by droplet counter current chromatography (DCCC) coupled with high-performance liquid chromatography (HPLC) and electrospray ionisation mass spectrometry (ESI-MS) data. By nuclear magnetic resonance (NMR) [1 H, 13 C and two-dimensional (2D) experiments] pure components were identified. Wound healing and cell proliferation assays were utilised to determine the role of the isolated S. tectorum (SVT) fraction on cellular migration and proliferation. The signalling pathways elicited from the SVT fractions, were analysed by Western blot analysis. Results In this study two rare natural components were identified, namely monosaccharide sedoheptulose and polyalcohol 2-C-methyl-D-erythritol, along with known organic acids and flavonoids. The fractions with high level of sedoheptulose enhance the proliferation and the cellular migration of epithelial HCT 116 cells. The intracellular signalling cascades elicited from the purified fractions induce the c-Src-mediated transactivation of EGFR and the activation of the STAT3 pathway which, in turn, are crucially involved in the cellular proliferation and migration. Conclusions Our study demonstrates the efficacy of purified fractions of S. tectorum L. in enhancing cellular proliferation and migration, suggesting their potential role as topical therapeutic treatments for wound healing.
Databáze: OpenAIRE
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