Expression and methylation of Dickkopf-1 in the pathogenesis and malignant transformation of oral submucous fibrosis
| Autor: | Xiaoshan Wu, Wenrui Zhang, Xiufang He, Yiting Guo, Chunjiao Xu, Alisha Stha, Miao-Miao Wang, Yumei Sun |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
animal structures Oral Submucous Fibrosis Methylation Protein expression Pathology and Forensic Medicine Malignant transformation Pathogenesis 03 medical and health sciences 0302 clinical medicine Medicine Humans Gene business.industry Wnt signaling pathway 030206 dentistry medicine.disease body regions stomatognathic diseases Cell Transformation Neoplastic Otorhinolaryngology Oral submucous fibrosis 030220 oncology & carcinogenesis embryonic structures DNA methylation Cancer research Carcinoma Squamous Cell Periodontics Mouth Neoplasms Oral Surgery business |
| Zdroj: | Journal of oral pathologymedicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral PathologyREFERENCES. 49(8) |
| ISSN: | 1600-0714 |
| Popis: | Background Dickkopf-1 is an inhibitor of the Wnt/β-catenin pathway, but the role of Dickkopf-1 in oral submucous fibrosis remains unclear. We evaluated the protein expression and gene methylation levels of dickkopf-1 to determine the mechanism underlying abnormal Wnt/β-catenin pathway activation. Methods Healthy mucosa, oral submucous fibrosis, oral squamous cell carcinoma, and cancer-adjacent tissues were collected. The expression and promoter methylation levels of dickkopf-1 were analyzed. Results The expression levels of dickkopf-1 in oral submucous fibrosis and oral squamous cell carcinoma tissues were lower than those in healthy and cancer-adjacent tissues. The methylation levels of the dickkopf-1 gene in oral submucous fibrosis and oral squamous cell carcinoma tissues were higher than those in healthy and cancer-adjacent tissues. Dickkopf-1 expression was negatively correlated with dickkopf-1 gene methylation. Conclusions High dickkopf-1 methylation levels in oral submucous fibrosis and oral squamous cell carcinoma tissues may decrease dickkopf-1 expression, which may induce an abnormal activation of the Wnt/β-catenin pathway and oral submucous fibrosis pathogenesis. |
| Databáze: | OpenAIRE |
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