Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
| Autor: | Luoyingzi Xie, Zhirong Li, Lifan Xu, Lilin Ye, Xiangyu Chen, Qizhao Huang, Leiqiong Gao, Qiao Liu, Qing Wu, Qin Tian, Shuqiong Wen, Junyi Guo, Yiding Li, Zhiming Wang, Li Hu, Jianfang Tang, Lisha Wang, Huayu Lin, Juan Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Tumor microenvironment
General Immunology and Microbiology biology General Chemical Engineering General Neuroscience T cell Major histocompatibility complex medicine.disease_cause General Biochemistry Genetics and Molecular Biology Immune system medicine.anatomical_structure Immunity biology.protein medicine Cancer research Cytotoxic T cell Carcinogenesis CD8 |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/62442 |
| Popis: | T cell-mediated immunity plays a crucial role in immune responses against tumors, with cytotoxic T lymphocytes (CTLs) playing the leading role in eradicating cancerous cells. However, the origins and replenishment of tumor antigen-specific CD8+ T cells within the tumor microenvironment (TME) remain obscure. This protocol employs the B16F10-OVA melanoma cell line, which stably expresses the surrogate neoantigen, ovalbumin (OVA), and TCR transgenic OT-I mice, in which over 90% of CD8+ T cells specifically recognize the OVA-derived peptide OVA257-264 (SIINFEKL) bound to the class I major histocompatibility complex (MHC) molecule H2-Kb. These features enable the study of antigen-specific T cell responses during tumorigenesis. Combining this model with tumor transplantation surgery, tumor tissues from donors were transplanted into tumor-matched syngeneic recipient mice to precisely trace the influx of recipient-derived immune cells into transplanted donor tissues, allowing the analysis of the immune responses of tumor-inherent and periphery-originated antigen-specific CD8+ T cells. A dynamic transition was found to occur between these two populations. Collectively, this experimental design has provided another approach to precisely investigate the immune responses of CD8+ T cells in TME, which will shed new light on tumor immunology. |
| Databáze: | OpenAIRE |
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