Variation of virulence of five Aspergillus fumigatus isolates in four different infection models
| Autor: | Keizer, E M, Valdes, I D, Forn-Cuni, G, Klijn, E, Meijer, A H, Hillman, F, Wösten, H A B, de Cock, H, Sub Molecular Microbiology, Molecular Microbiology |
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| Přispěvatelé: | Sub Molecular Microbiology, Molecular Microbiology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Life Cycles
Embryology Single Nucleotide Polymorphisms Aspergillosis Pathology and Laboratory Medicine Epithelium Conidium Aspergillus fumigatus Larvae Animal Cells Fungal Reproduction Medicine and Health Sciences Fungal Spore Germination Amoebas Zebrafish Fungal Pathogens Protozoans 0303 health sciences Multidisciplinary Strain (chemistry) Virulence Eukaryota Animal Models 3. Good health Phenotype Aspergillus Aspergillus Fumigatus Experimental Organism Systems Fungal Molds Medical Microbiology Osteichthyes Vertebrates Medicine Allergic bronchopulmonary aspergillosis Pathogens Cellular Types Anatomy Research Article Science Mycology Biology Research and Analysis Methods Microbiology Amoeba (operating system) 03 medical and health sciences Dogs Model Organisms medicine Genetics Animals General Gene Microbial Pathogens 030304 developmental biology 030306 microbiology Embryos Organisms Fungi Correction Biology and Life Sciences Epithelial Cells Cell Biology medicine.disease biology.organism_classification Fish Biological Tissue Mutation Animal Studies Zoology Developmental Biology |
| Zdroj: | PLoS ONE, 16(7). PUBLIC LIBRARY SCIENCE PLoS ONE PLoS One, 16(7). Public Library of Science PLoS ONE, Vol 16, Iss 7, p e0252948 (2021) PLoS ONE, 16(7) |
| ISSN: | 1932-6203 |
| Popis: | Conidia of Aspergillus fumigatus are inhaled by humans on daily basis. As a consequence, these conidia can cause infections that differ in severity ranging from allergic bronchopulmonary aspergillosis to invasive aspergillosis. In this study we compared virulence of five A. fumigatus isolates in four different infection models to address the predictive value of different model systems. Two of the A. fumigatus strains were isolated from dogs with a non-invasive sino-nasal aspergillosis (DTO271-B5 and DTO303-F3), while three strains were isolated from human patients with invasive aspergillosis (Af293, ATCC46645 and CEA10). Infection models used encompassed cultured type II A549 lung epithelial cells, Protostelium aurantium amoeba, Galleria melonella larvae and zebrafish embryos. No major differences in virulence between these five strains were observed in the lung epithelial cell model. In contrast, strain ATCC46645 was most virulent in the amoeba and zebrafish model, whereas it was much less virulent in the Galleria infection model. DTO303-F3 was most virulent in the latter model. In general, reference strain Af293 was less virulent as compared to the other strains. Genome sequence analysis showed that this latter strain differed from the other four strains in 136 SNPs in virulence-related genes. Together, our results show that virulence of individual A. fumigatus strains show significant differences between infection models. We conclude that the predictive value of different model systems varies since the relative virulence across fungal strains does not hold up across different infection model systems. |
| Databáze: | OpenAIRE |
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