Immunocytochemical demonstration of dopamine-beta-hydroxylase and cytochrome B561 on the axonal reticulum in bovine sympathetic neurons
| Autor: | W P De Potter, B. Miserez, J. Quatacker, W. G. Annaert |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Sympathetic Nervous System
Histology Blotting Western Immunocytochemistry Dopamine beta-Hydroxylase Biology symbols.namesake medicine Animals Neurons Cytochrome b561 Endoplasmic reticulum Vas deferens Phosphotungstic Acid Immunogold labelling Golgi apparatus Cytochrome b Group Immunohistochemistry Axons Cell biology medicine.anatomical_structure nervous system Biochemistry symbols Cattle Electrophoresis Polyacrylamide Gel Neuron Anatomy Reticulum |
| Zdroj: | The journal of histochemistry and cytochemistry |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/40.10.1527378 |
| Popis: | In sympathetic neurons the axonal reticulum can be considered an extension of the secretory pole of the Golgi apparatus. If this tubular system indeed represents the neurosecretory apparatus, it would likely contain on its membranes the enzymes involved in catecholamine synthesis. To test this hypothesis, we investigated the distribution of dopamine-beta-hydroxylase and cytochrome b561 in bovine splenic nerve and nerve terminals in the vas deferens with an immunogold procedure after glycolmethacrylate embedding. Counterstaining with phosphotungstic acid at low pH selectively revealed the axonal reticulum elements. With antibodies against both enzymes, gold labeling was observed over the large dense-cored vesicles, the Golgi-associated axonal reticulum, the reticulum within axons, and the tubular complex at the nerve terminal. From our results it can be concluded that in sympathetic neurons the axonal reticulum represents a tubular neurosecretory system, extending from the Golgi apparatus in the cell soma to the nerve terminal. This concept emphasizes the local production of neurosecretory vesicles and may be of importance in the interpretation of neuronal transmission in normal and diseased states. |
| Databáze: | OpenAIRE |
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