Selective recognition of creatinine – Development of a colorimetric sensor
Autor: | Unni Sivasankaran, Krishnapillai Girish Kumar, Theresa Jos |
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Rok vydání: | 2018 |
Předmět: |
Materials science
Biophysics Metal Nanoparticles Nanoparticle 02 engineering and technology engineering.material 01 natural sciences Biochemistry chemistry.chemical_compound Colorimetric sensor Humans Copper nanoparticle Cysteine Particle Size Colorimetry Molecular Biology Renal biomarkers Creatinine Chromatography Molecular Structure Dynamic range 010401 analytical chemistry Cell Biology 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry engineering Noble metal 0210 nano-technology Copper |
Zdroj: | Analytical Biochemistry. 544:1-6 |
ISSN: | 0003-2697 |
Popis: | The present report describes a simple and cost effective protocol for colourimetric determination of creatinine (CR), L-cysteine stabilized copper nanoparticles (L-cys-CuNPs) exhibited selective and sensitive interaction with CR. Utilizing this interaction, a colourimetric sensor has been developed based on the reduction in LSPR intensity as monitored by a UV–visible spectrophotometer. The developed sensor exhibited a linear dynamic range of 5.33 × 10−6 to 3.33 × 10−7 M. Proposed sensor is simple and cost - effective compared to methods based on noble metal nanoparticles and the sensitivity to determine CR was as low as 4.54 × 10−10 M. The sensor was successfully applied for quantification of CR in artificial serum and urine samples. Sensor developed in this work has a high potential for rapid and on-site determination of CR in physiological and clinical samples. |
Databáze: | OpenAIRE |
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