Facial granulomatous diseases: a study of four cases tested for the presence of Mycobacterium tuberculosis DNA using nested polymerase chain reaction
| Autor: | G. Ferrara, Maria Cannone, Massino C. Barberis, Gaetano De Rosa, Massimiliano Scalvenzi, Stefania Staibano, Mario Delfino |
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| Přispěvatelé: | Ferrara, G, Cannone, M, Scalvenzi, Massimiliano, Delfino, M, Staibano, Stefania, DE ROSA, Gaetano, Barberis, Mc |
| Rok vydání: | 2001 |
| Předmět: |
Male
Pathology Antitubercular Agents microbiology/pathology Female Granulomatous Disease Differential Drug Therapy Granulomatous Disease Chronic Polymerase Chain Reaction law.invention law therapeutic use DNA Chronic therapeutic use Male Middle Aged Mycobacterium tuberculosi Polymerase chain reaction diagnosis/drug therapy/microbiology Granulomatous Rosacea biology diagnosis/drug therapy/microbiology Tuberculosi Bacterial General Medicine Middle Aged Cutaneou Granuloma Drug Therapy Combination Female Rifampin Epithelioid cell Combination Ethambutol Ethambutol Adult DNA Bacterial medicine.medical_specialty Tuberculosis Adult Antitubercular Agent Dermatology Pathology and Forensic Medicine Mycobacterium tuberculosis Diagnosis Differential analysis Diagnosi medicine Isoniazid Humans Tuberculosis Cutaneous business.industry therapeutic use Face medicine.disease biology.organism_classification Rosacea drug therapy/microbiology/pathology Humans Isoniazid genetics/isolation /&/ purification Polymerase Chain Reaction Rifampin Face therapeutic use Rosacea business Nested polymerase chain reaction |
| Zdroj: | The American Journal of dermatopathology. 23(1) |
| ISSN: | 0193-1091 |
| Popis: | The histopathologic diagnosis of cutaneous tuberculosis (CTB) is often troublesome, because there are several other entities (tuberculids, demodicidosis, granulomatous rosacea, and acne agminata) that may display granulomatous inflammation with caseation necrosis. The current study describes four cases of granulomatous disease of the face. The final diagnosis (assessed on the basis of the clinical response to therapy) was CTB in three cases and granulomatous rosacea in one case. Histologically, epithelioid granulomas were a constant feature; in one case of CTB, they displayed a palisading (granuloma annulare-like) arrangement. Caseation necrosis was a prominent feature only in the case of granulomatous rosacea. Routinely processed biopsy specimens were evaluated with nested polymerase chain reaction (nPCR) for Mycobacterium tuberculosis (MBT) DNA. The correlation between nPCR results and clinical outcome was less than optimal; in fact, one case showed an excellent clinical response to the antituberculous drug therapy despite the absence of MBT DNA amplification. In granulomatous diseases of the face, the importance of evaluating not only nPCR but the overall clinicopathologic picture so as to avoid diagnostic misinterpretations is emphasized. |
| Databáze: | OpenAIRE |
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