Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection
| Autor: | Ana L. Moldoveanu, Carolyn M. Nielsen, Alansana Darboe, Ana Rodriguez-Galan, Matthew J. White, Chiara Lusa, Ron H Behrens, Martin R. Goodier, Eleanor M. Riley |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine Interleukin 2 Human cytomegalovirus viruses medicine.medical_treatment Secondary infection Innate Immunity and Inflammation Immunology Orthomyxoviridae Cytomegalovirus Biology Lymphocyte Activation Interferon-gamma Young Adult 03 medical and health sciences Interleukin 21 0302 clinical medicine Immune system Influenza Human Journal Article medicine Humans Immunology and Allergy Interferon gamma Cells Cultured Research Support Non-U.S. Gov't Vaccination virus diseases Middle Aged biology.organism_classification medicine.disease Virology 3. Good health Killer Cells Natural 030104 developmental biology Cytokine Influenza Vaccines Cytomegalovirus Infections Interleukin-2 Immunologic Memory 030215 immunology medicine.drug |
| Zdroj: | Goodier, M R, Rodriguez-Galan, A, Lusa, C, Nielsen, C M, Darboe, A, Moldoveanu, A L, White, M J, Behrens, R & Riley, E M 2016, ' Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells : Impact of Human Cytomegalovirus Infection ', Journal of Immunology, vol. 197, no. 1, pp. 313-25 . https://doi.org/10.4049/jimmunol.1502049 The Journal of Immunology Author Choice |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Human NK cells are activated by cytokines, immune complexes, and signals transduced via activating ligands on other host cells. After vaccination, or during secondary infection, adaptive immune responses can enhance both cytokine-driven and Ab-dependent NK cell responses. However, induction of NK cells for enhanced function after in vitro exposure to innate inflammatory cytokines has also been reported and may synergize with adaptive signals to potentiate NK cell activity during infection or vaccination. To test this hypothesis, we examined the effect of seasonal influenza vaccination on NK cell function and phenotype in 52 previously unvaccinated individuals. Enhanced, IL-2–dependent, NK cell IFN-γ responses to Influenza A/California/7/2009 virus were detected up to 4 wk postvaccination and higher in human CMV (HCMV)-seronegative (HCMV−) individuals than in HCMV-seropositive (HCMV+) individuals. By comparison, robust NK cell degranulation responses were observed both before and after vaccination, due to high titers of naturally occurring anti-influenza Abs in human plasma, and did not differ between HCMV+ and HCMV− subjects. In addition to these IL-2–dependent and Ab-dependent responses, NK cell responses to innate cytokines were also enhanced after influenza vaccination; this was associated with proliferation of CD57− NK cells and was most evident in HCMV+ subjects. Similar enhancement of cytokine responsiveness was observed when NK cells were cocultured in vitro with Influenza A/California/7/2009 virus, and this was at least partially dependent upon IFN-αβR2. In summary, our data indicate that attenuated or live viral vaccines promote cytokine-induced memory-like NK cells and that this process is influenced by HCMV infection. |
| Databáze: | OpenAIRE |
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