Independent chondrogenic potential of canine bone marrow-derived mesenchymal stem cells in monolayer expansion cultures decreases in a passage-dependent pattern
| Autor: | Hm Suranji Wijekoon, Jing Fang, Sangho Kim, Eugene C Bwalya, Masahiro Okumura, Kenji Hosoya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
phenotype Type II collagen Cell Culture Techniques Dexamethasone Andrology Transforming Growth Factor beta1 03 medical and health sciences 0302 clinical medicine Dogs bone marrow-derived mesenchymal stem cell Gene expression chondrogenesis medicine Animals Serial Passage Aggrecan Cell Proliferation General Veterinary Full Paper Chemistry Mesenchymal stem cell monolayer culture Mesenchymal Stem Cells Chondrogenesis Phenotype 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis dog Surgery Bone marrow Stem cell |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 0916-7250 |
| Popis: | Although chondroinductive growth factors are considered necessary for chondrogenesis of bone marrow-derived mesenchymal stem cells (BMSC), independent and spontaneous chondrogenesis has been previously demonstrated in adult horses, bovine calves and adult human BMSC. Surprisingly, adult canine BMSC under similar culture conditions previously failed to demonstrate chondrogenesis. The present study evaluated independent chondrogenic potential of BMSC sourced from three young dogs in the absence of known chondroinductive factors. BMSC were culture expanded in 10% DMEM up to third passage (P3). At each passage, the phenotype of BMSC was evaluated by RT-PCR gel electrophoresis and qPCR. BMSC exhibited a chondrogenic phenotype in the absence of dexamethasone and TGF-beta 1 as verified by the expression of Sox-9, type II collagen and aggrecan. Sox-9 was significantly downregulated (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|