Unveiling sugarcane defense response to Mythimna separata herbivory by a combination of transcriptome and metabolic analyses

Autor: Hua-Ying Fu, Yuan Xie, Jinda Wang, You-Ming Hou, San-Ji Gao, Jia-song Zhang, Ya-Ru Wang, Ran Wang
Rok vydání: 2021
Předmět:
Zdroj: Pest Management Science. 77:4799-4809
ISSN: 1526-4998
1526-498X
DOI: 10.1002/ps.6526
Popis: Background Sugarcane is the most important sugar crops in the world. Like other crops, sugarcane is also suffered from herbivorous insect attack. The oriental armyworm Mythimna separata is a devastating pest of various crop in northeast Asia, outbreak of this pest resulted in substantially yield loss including sugarcane. However, the plant defense response situation is widely acquisition in model crops, but little information about how sugarcane plants defend themselves against this herbivore at the molecular and biochemical levels is studied. Results We combined transcriptome and metabolomics analysis to investigate the changes in gene expression and metabolic processes that occurred in sugarcane plants after continuous feeding by M. separata larvae for 12, and 24 h. We identified 13 662 genes and 55 metabolites that were differentially regulated in sugarcane plants fed upon by M. separata. The genes involved in phytohormones, transcription factors, and kinase-related were activated and the metabolism compounds such as carbohydrate, amino acid, ferulic substances and glutathione were detected regulated in sugarcane defense response. Comparable analyses showed a close correspondence relationship among pathways of phenylalanine metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis in transcript and metabolite profiles. Furthermore, a bioassay experiment was conducted to test the influence of up-regulated metabolites on M. separata growth and found chlorogenic acid showed lethal effect. Conclusion The results of our study greatly enhanced understanding on sugarcane induced defense response mechanism against herbivore infestation at the transcriptional and metabolic levels. Also make contributions to provide clues for development of green pest control method. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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