Completion of replication map of Saccharomyces cerevisiae chromosome III
| Autor: | Jitakshi De, Carol S. Newlon, Ann Dershowitz, Arkadi Poloumienko |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Genetics
DNA Replication biology Autonomously replicating sequence Saccharomyces cerevisiae DNA replication Chromosome Chromosome Mapping Eukaryotic DNA replication Replication Origin Cell Biology biology.organism_classification Origin of replication Article Control of chromosome duplication Origin recognition complex Chromosomes Fungal Cloning Molecular Molecular Biology |
| Zdroj: | Molecular biology of the cell. 12(11) |
| ISSN: | 1059-1524 |
| Popis: | In Saccharomyces cerevisiae chromosomal DNA replication initiates at intervals of approximately 40 kb and depends upon the activity of autonomously replicating sequence (ARS) elements. The identification of ARS elements and analysis of their function as chromosomal replication origins requires the use of functional assays because they are not sufficiently similar to identify by DNA sequence analysis. To complete the systematic identification of ARS elements on S. cerevisiae chromosome III, overlapping clones covering 140 kb of the right arm were tested for their ability to promote extrachromosomal maintenance of plasmids. Examination of chromosomal replication intermediates of each of the seven ARS elements identified revealed that their efficiencies of use as chromosomal replication origins varied widely, with four ARS elements active inor = 10% of cells in the population and two ARS elements active inor = 90% of the population. Together with our previous analysis of a 200-kb region of chromosome III, these data provide the first complete analysis of ARS elements and DNA replication origins on an entire eukaryotic chromosome. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|