Vestitol Isolated from Brazilian Red Propolis Inhibits Neutrophils Migration in the Inflammatory Process: Elucidation of the Mechanism of Action
| Autor: | Fernanda V. S. Castanheira, Pedro Luiz Rosalen, Marcos Guilherme da Cunha, Thiago M. Cunha, David E. Cólon, Severino Matias de Alencar, Bruno Bueno-Silva, Marcelo Franchin |
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| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Neutrophils Chemokine CXCL1 Anti-Inflammatory Agents Nitric Oxide Synthase Type II Pharmaceutical Science Pharmacology Propolis Analytical Chemistry Mice 03 medical and health sciences Isoflavonoid In vivo ÓXIDO NÍTRICO Drug Discovery medicine Animals Macrophage Flavonoids Mice Inbred BALB C Molecular Structure Chemistry Organic Chemistry Chemotaxis In vitro CXCL1 030104 developmental biology Neutrophil Infiltration Complementary and alternative medicine Mechanism of action Immunology Molecular Medicine medicine.symptom Brazil |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1520-6025 0163-3864 |
| Popis: | Vestitol is an isoflavonoid isolated from Brazilian red propolis with potential anti-inflammatory activity. This study investigated the mechanism of action of vestitol on the modulation of neutrophil migration in the inflammatory process. Pre-treatment with vestitol at 1, 3, or 10 mg/kg reduced LPS- or mBSA-induced neutrophil migration and the release of CXCL1/KC and CXCL2/MIP-2 in vivo. Likewise, pre-treatment with vestitol at 1, 3, or 10 μM reduced the levels of CXCL1/KC and CXCL2/MIP-2 in macrophage supernatants in vitro. Moreover, the administration of vestitol (10 mg/kg) reduced leukocyte rolling and adherence in the mesenteric microcirculation of mice. The pre-treatment with vestitol (10 mg/kg) in iNOS(-/-) mice did not block its activity concerning neutrophil migration. With regard to the activity of vestitol on neutrophils isolated from the bone marrow of mice, there was a reduction on the chemotaxis of CXCL2/MIP-2 or LTB4-induced neutrophils and on calcium influx after pre-treatment with the compound at 3 or 10 μM. There was no change in CXCR2 expression by neutrophils treated with vestitol at 10 μM. These findings demonstrate that vestitol is a promising novel anti-inflammatory agent. |
| Databáze: | OpenAIRE |
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