Listeria spp. Isolated from Tonsils of Wild Deer and Boars: Genomic Characterization

Autor: Carla Palacios-Gorba, Juan J. Quereda, Marc Lecuit, Jesús Gomis, María L. Mocé, Ángel Gómez-Martín, Alexandre Leclercq, E. Jiménez-Trigos, Alexandra Moura
Přispěvatelé: Universidad Cardenal Herrera-CEU (CEU-UCH), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), This work was supported by Generalitat Valenciana (project reference GV/2018/A/183) and by the Spanish Ministry of Science and Innovation (project reference PID2019-110764RA-I00) (J.J.Q.), Institut Pasteur, Inserm, and Santé Publique France. J. J. Quereda is supported by a 'Ramón y Cajal' contract of the Spanish Ministry of Science, Innovation and Universities (RYC-2018-024985-I). C. Palacios-Gorba is supported by a predoctoral contract from the Universidad Cardenal Herrera-CEU., We thank Hélène Bracq-Dieye, Nathalie Tessaud-Rita, Pierre Thouvenot, and Guillaume Vales (Institut Pasteur, Paris, France) for DNA extraction and the P2M platform (Institut Pasteur, Paris, France) for genome sequencing., DIAKITE, andrée, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
[SDV.BA] Life Sciences [q-bio]/Animal biology
[SDV]Life Sciences [q-bio]
medicine.disease_cause
MESH: Genome
Bacterial
Applied Microbiology and Biotechnology
MESH: Listeria
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
MESH: Animals
MESH: Phylogeny
Pathogen
2. Zero hunger
0303 health sciences
education.field_of_study
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
Ecology
Public and Environmental Health Microbiology
[SDV.BA]Life Sciences [q-bio]/Animal biology
MESH: Feces
3. Good health
wild animal
[SDV] Life Sciences [q-bio]
MESH: Multilocus Sequence Typing
MESH: Palatine Tonsil
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Listeria ivanovii
MESH: Whole Genome Sequencing
Biotechnology
Population
MESH: Deer
Biology
Microbiology
03 medical and health sciences
Listeria monocytogenes
medicine
education
Feces
030304 developmental biology
030306 microbiology
biology.organism_classification
Pathogenicity island
MESH: Sus scrofa
virulence
MESH: Listeriosis
tonsil
Listeria
Multilocus sequence typing
cgMLST
WGS
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Food Science
Zdroj: Applied and Environmental Microbiology
Applied and Environmental Microbiology, 2021, 87 (6), pp.e02651-20. ⟨10.1128/AEM.02651-20⟩
Appl Environ Microbiol
Applied and Environmental Microbiology, American Society for Microbiology, 2021, 87 (6), pp.e02651-20. ⟨10.1128/AEM.02651-20⟩
ISSN: 0099-2240
1098-5336
Popis: Listeria monocytogenes is a major human and animal foodborne pathogen. However, data from environmental reservoirs remain scarce. Here, we used whole-genome sequencing to characterize Listeria species isolates recovered over 1 year from wild animals in their natural habitats in Spain. Three different Listeria spp. (L. monocytogenes [n = 19], Listeria ivanovii subsp. londoniensis [n = 4], and Listeria innocua [n = 3]) were detected in 23 animal tonsils (9 deer, 14 wild boars) and 2 feeding troughs. No Listeria species was detected in feces. L. monocytogenes was detected in tonsils of 44.4% (8 out of 18) of deer and 40.7% (11 out of 27) of wild boars. L. monocytogenes isolates belonged to 3 different core genome multilocus sequence typing (cgMLST) types (CTs) of 3 distinct sublineages (SL1, SL387, and SL155) from lineages I and II. While cgMLST type L1-SL1-ST1-CT5279 (IVb; clonal complex 1 [CC1]) occurred only in one animal, types L1-SL387-ST388-CT5239 (IVb; CC388) and L2-SL155-ST155-CT1170 (IIa; CC155) were retrieved from multiple animals. In addition, L1-SL387-ST388-CT5239 (IVb; CC388) isolates were collected 1 year apart, revealing their long-term occurrence within the animal population and/or environmental reservoir. The presence of identical L. monocytogenes strains in deer and wild boars suggests contamination from a common food or environmental source, although interhost transmission cannot be excluded. Pathogenicity islands LIPI-1, LIPI-3, and LIPI-4 were present in 100%, 5%, and 79% of the L. monocytogenes isolates, respectively, and all L. monocytogenes lineage II isolates (n = 3) carried SSI-1 stress islands. This study highlights the need for monitoring L. monocytogenes environmental contamination and the importance of tonsils as a possible L. monocytogenes intrahost reservoir. IMPORTANCEListeria monocytogenes is a foodborne bacterial pathogen responsible for listeriosis. Whole-genome sequencing has been extensively used in public health and food industries to characterize circulating Listeria isolates, but genomic data on isolates occurring in natural environments and wild animals are still scarce. Here, we show that wild animals carry pathogenic Listeria and that the same genotypes can be found at different time points in different host species. This work highlights the need of Listeria species monitoring of environmental contamination and the importance of tonsils as a possible L. monocytogenes intrahost reservoir.
Databáze: OpenAIRE
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