Lipase-catalyzed biodiesel synthesis with different acyl acceptors
| Autor: | D Nevena Ognjanovic, V Svetlana Saponjic, D Zorica Knezevic, I Dejan Bezbradica |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Immobilized enzyme
020209 energy biodiesel n-butanol 02 engineering and technology 01 natural sciences 7. Clean energy complex mixtures chemistry.chemical_compound 2-propanol lcsh:Technology (General) 0202 electrical engineering electronic engineering information engineering Glycerol lipase Organic chemistry Lipase Triacetin methanol Biodiesel biology 010405 organic chemistry Chemistry General Engineering food and beverages Transesterification 0104 chemical sciences methyl acetate Biodiesel production biology.protein lcsh:T1-995 Methanol |
| Zdroj: | Acta Periodica Technologica, Vol 2008, Iss 39, Pp 161-169 (2008) Acta periodica technologica |
| ISSN: | 1450-7188 |
| Popis: | Biodiesel is an alternative fuel for diesel engine that is environmentally acceptable. Conventionally, biodiesel is produced by transesterification of triglycerides and short alcohols in the presence of an acid or an alkaline catalyst. There are several problems associated with this kind of production that can be resolved by using lipase as the biocatalyst. The aim of the present work was to investigate novel acyl acceptors for biodiesel production. 2-Propanol and n-butanol have a less negative effect on lipase stability, and they also improve low temperature properties of the fuel. However, excess alcohol leads to inactivation of the enzyme, and glycerol, a major byproduct, can block the immobilized enzyme, resulting in low enzymatic activity. This problem was solved by using methyl acetate as acyl acceptor. Triacetylglycerol is produced instead of glycerol, and it has no negative effect on the activity of the lipase. U ovom radu ispitana je mogućnost primene različitih acil akceptora kao polaznih reaktanata umesto metanola u sintezi biodizela u cilju smanjenja inhibicije enzima, povećanja njegove stabilnosti i mogućnosti značajnog pojednostavljivanja izvođenja procesa. Prvo je ispitana mogućnost korišćenja različitih alkohola: 2-propanol i n-butanol koji imaju manji uticaj na denaturaciju enzima. Osim toga, primenom ovih alkohola dobija se gorivo boljeg kvaliteta, pošto se sa povećanjem broja ugljenikovih atoma povećava oktanski broj, temperatura paljenja, kao i sadržaj toplote. Drugi deo istraživanja bio je orijentisan ka ispitivanju mogućnosti korišćenja metilacetata kao pogodnog nukleofila. Za razliku od alkohola, primenom ovog acil akceptora omogućen je jednostepeni postupak sinteze biodizela, što značajno smanjuje vreme trajanja reakcije, kao i kompleksnost iste. Takođe, primenom metilacetata povećava se stabilnost biokatalizatora, što omogućava višekratnu upotrebu. Naime, u reakciji sa metilacetatom kao nusprodukt se ne stvara glicerol već triacetilglicerol, koji nema negativan efekat na stabilnost lipaze. |
| Databáze: | OpenAIRE |
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