7-hydroxytropolone is the main metabolite responsible for the fungal antagonism of Pseudomonas donghuensis strain SVBP6
| Autor: | Betina Cecilia Agaras, Marco Masi, Angela Tuzi, Antonio Evidente, Claudio Valverde, Federico Matías Muzio |
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| Přispěvatelé: | Muzio, F. M., Agaras, B. C., Masi, M., Tuzi, A., Evidente, A., Valverde, C. |
| Rok vydání: | 2019 |
| Předmět: |
Antifungal Agents
Transcription Factor Metabolite Pseudomonas donghuensis Argentina Bacterial Protein Transposases Biology Pseudomona Mutagenesi Microbiology Tropolone purl.org/becyt/ford/1 [https] 03 medical and health sciences chemistry.chemical_compound Ascomycota Bacterial Proteins Pseudomonas Antibiosis Antibiosi Antifungal Agent purl.org/becyt/ford/1.6 [https] Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Strain (chemistry) 030306 microbiology biology.organism_classification chemistry Macrophomina phaseolina Mutagenesis Antifungico 7-hidroxitropolona Antagonism Transposase Transcription Factors |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1462-2920 |
| Popis: | Pseudomonas donghuensis strain SVBP6, an isolate from an agricultural plot in Argentina, displays a broad-spectrum and diffusible antifungal activity, which requires a functional gacS gene but could not be ascribed yet to known secondary metabolites typical of Pseudomonas biocontrol species. Here, we report that Tn5 mutagenesis allowed the identification of a gene cluster involved in both the fungal antagonism and the production of a soluble tropolonoid compound. The ethyl acetate extract from culture supernatant showed a dose-dependent inhibitory effect against the phytopathogenic fungus Macrophomina phaseolina. The main compound present in the organic extract was identified by spectroscopic and X-ray analyses as 7-hydroxytropolone (7HT). Its structure and tautomerism was confirmed by preparing the two key derivatives 2,3-dimethoxy- and 2,7-dimethoxy-tropone. 7HT, but not 2,3- or 2,7-dimethoxy-tropone, mimicked the fungal inhibitory activity of the ethyl acetate extract from culture supernatant. The activity of 7HT, as well as its production, was barely affected by the presence of up to 50 μM added iron (Fe+2). To summarize, P. donghuensis SVBP6 produces 7HT under the positive control of the Gac-Rsm cascade and is the main active metabolite responsible for the broad-spectrum inhibition of different phytopathogenic fungi. Fil: Muzio, Federico Matías. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Centro de Bioquimica y Microbiologia de Suelos. Laboratorio de Fisiologia, Genetica de Bacterias Para Plantas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Agaras, Betina Cecilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Centro de Bioquimica y Microbiologia de Suelos. Laboratorio de Fisiologia, Genetica de Bacterias Para Plantas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Masi, Marco. Università degli Studi di Napoli Federico II; Italia Fil: Tuzi, Angela. Università degli Studi di Napoli Federico II; Italia Fil: Evidente, Antonio. Università degli Studi di Napoli Federico II; Italia Fil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Centro de Bioquimica y Microbiologia de Suelos. Laboratorio de Fisiologia, Genetica de Bacterias Para Plantas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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