Ethanol Exposure Induces Upregulation of Specific MicroRNAs in Zebrafish Embryos
| Autor: | João Simões, Violeta Ferreira, Ana R. Bezerra, Patrícia M. Pereira, Ana R. Soares, Marisa Reverendo, Gabriela Moura, Manuel A. S. Santos |
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| Rok vydání: | 2012 |
| Předmět: |
Embryo
Nonmammalian Apoptosis Real-Time Polymerase Chain Reaction Toxicology 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation microRNA Transcriptional regulation Animals Gene Zebrafish 030304 developmental biology 0303 health sciences Ethanol biology Cell Cycle Abnormalities Drug-Induced Central Nervous System Depressants Gene Expression Regulation Developmental Embryo biology.organism_classification Molecular biology Up-Regulation Ethanol toxicity Cell biology MicroRNAs Zebrafish Toxicity Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1096-0929 1096-6080 |
| Popis: | Prenatal exposure to ethanol leads to a myriad of developmental disorders known as fetal alcohol spectrum disorder, often characterized by growth and mental retardation, central nervous system damage, and specific craniofacial dysmorphic features. The mechanisms of ethanol toxicity are not fully understood, but exposure during development affects the expression of several genes involved in cell cycle control, apoptosis, and transcriptional regulation. MicroRNAs (miRNAs) are implicated in some of these processes, however, it is not yet clear if they are involved in ethanol-induced toxicity. In order to clarify this question, we have exposed zebrafish embryos to ethanol and evaluated whether a miRNA deregulation signature could be obtained. Zebrafish embryos were exposed to 1 and 1.5% of ethanol from 4 h postfertilization (hpf) to 24 hpf. The miRNA expression profiles obtained reveal significant miRNA deregulation and show that both ethanol concentrations upregulate miR-153a, miR-725, miR-30d, let-7k, miR-100, miR-738, and miR-732. Putative gene targets of deregulated miRNAs are involved in cell cycle control, apoptosis, and transcription, which are the main processes affected by ethanol toxicity. The conservation of affected mechanisms among vertebrates leads us to postulate that similar miRNA deregulation occurs in humans, highlighting a relevant role of miRNAs in ethanol toxicology. published |
| Databáze: | OpenAIRE |
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