The DNA-Dependent Protein Kinase Participates in the Activation of NFκB Following DNA Damage
| Autor: | Subimal Basu, Brendan D. Price, Matthew B. Youmell, Kenneth R. Rosenzweig |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA damage
medicine.medical_treatment Biophysics DNA-Activated Protein Kinase Protein Serine-Threonine Kinases Biology Biochemistry Wortmannin chemistry.chemical_compound NF-KappaB Inhibitor alpha Tumor Cells Cultured medicine Humans Phosphorylation Nuclear protein Molecular Biology Transcription factor Etoposide Tumor Necrosis Factor-alpha Kinase NF-kappa B Nuclear Proteins Glioma Cell Biology Molecular biology Androstadienes DNA-Binding Proteins Cytokine chemistry Gamma Rays I-kappa B Proteins Tumor necrosis factor alpha DNA Damage HeLa Cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 247:79-83 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.8741 |
| Popis: | The NFkB transcription factor is activated by diverse stimuli, including Ionizing Radiation (IR) and the cytokine TNF alpha. The role of DNA-PK, a protein kinase involved in the response to DNA damage, in the activation of NF kappa B by IR and TNF alpha was examined. In M059K cells, which express DNA-PK, NF kappa B was activated by both TNF alpha and IR. In M059J cells, which do not express DNA-PK, IR did not activate NF kappa B, whereas TNF alpha induction of NF kappa B was still observed. In HeLa cells, wortmannin, an inhibitor of DNA-PK, blocked the induction of NF kappa B by IR but not by TNF alpha. DNA-PK also phosphorylated the NF kappa B inhibitory proteins IkB-alpha and IkB-beta in vitro, and deletion analysis demonstrated that DNA-PK phosphorylates 2 distinct regions of IkB-beta. These results indicate that DNA-PK participates in the activation of NF kappa B by IR but not by TNF alpha. |
| Databáze: | OpenAIRE |
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