A Multiomic Approach to Understand How Pleurotus eryngii Transforms Non-Woody Lignocellulosic Material
| Autor: | Igor V. Grigoriev, Ana Gutiérrez, Anna Lipzen, Gisela Marques, Delphine Chaduli, Rashid Babiker, Teeratas Kijpornyongpan, Ander Peña, Susana Camarero, Marie-Noëlle Rosso, Ángel T. Martínez, Jorge Rencoret, Davinia Salvachúa, Francisco J. Ruiz-Dueñas, María Isabel Sánchez-Ruiz, Mei Wang, Vivian Ng, Mansi Chovatia |
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| Přispěvatelé: | Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This research was funded by the GENOBIOREF (BIO2017-86559-R) project of the Spanish Ministry of Science and Innovation (co-financed by FEDER funds), and by the Consejo Superior de Investigaciones Científicas (PIE-202120E019 and PIE-201620E081)., Ministerio de Ciencia e Innovación (España), European Commission, Biological and Environmental Research (US), Department of Energy (US), Consejo Superior de Investigaciones Científicas (España), Rencoret, Jorge [0000-0003-2728-7331], Marques, Gisela [0000-0002-6431-8267], Gutiérrez Suárez, Ana [0000-0002-8823-9029], Grigoriev, Igor V. [0000-0002-3136-8903], Rosso, Marie-Noëlle [0000-0001-8317-7220], Martínez, Ángel T. [0000-0002-1584-2863], Ruiz-Dueñas, F. J. [0000-0002-9837-5665], Rencoret, Jorge, Marques, Gisela, Gutiérrez Suárez, Ana, Grigoriev, Igor V., Rosso, Marie-Noëlle, Martínez, Ángel T., Ruiz-Dueñas, F. J. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Proteomics Cellobiose dehydrogenase solid-state fermentation QH301-705.5 lignocellulose transformation Plant Science Polysaccharide wheat-straw Article Pleurotus eryngii 03 medical and health sciences chemistry.chemical_compound transcriptomics proteomics Solid-state fermentation Oxidative enzyme Lignin Metabolomics Biology (General) Cellulose Transcriptomics Ecology Evolution Behavior and Systematics 030304 developmental biology chemistry.chemical_classification Laccase 0303 health sciences biology 030306 microbiology Lignin-modifying enzymes Wheat–straw ligninmodifying enzymes biology.organism_classification metabolomics Lignocellulose transformation oxidoreductases Enzyme [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Biochemistry carbohydrate-active enzymes Carbohydrate-active enzymes lignin-modifying enzymes wheat–straw Oxidoreductases |
| Zdroj: | Journal of Fungi Volume 7 Issue 6 Journal of Fungi, 2021, 7 (6), ⟨10.3390/jof7060426⟩ Digital.CSIC. Repositorio Institucional del CSIC instname Journal of fungi (Basel, Switzerland), vol 7, iss 6 Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Journal of Fungi, Vol 7, Iss 426, p 426 (2021) |
| ISSN: | 2309-608X |
| DOI: | 10.3390/jof7060426 |
| Popis: | 31 páginas.- 14 figuras.- 92 referencias.- Supplementary Materials: The following are available online at https://www.mdpi.com/article/10.3390/jof7060426/s1 .- Acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Pleurotus eryngii is a grassland-inhabiting fungus of biotechnological interest due to its ability to colonize non-woody lignocellulosic material. Genomic, transcriptomic, exoproteomic, and metabolomic analyses were combined to explain the enzymatic aspects underlaying wheat–straw transformation. Up-regulated and constitutive glycoside–hydrolases, polysaccharide–lyases, and carbohydrate–esterases active on polysaccharides, laccases active on lignin, and a surprisingly high amount of constitutive/inducible aryl–alcohol oxidases (AAOs) constituted the suite of extracellular enzymes at early fungal growth. Higher enzyme diversity and abundance characterized the longer-term growth, with an array of oxidoreductases involved in depolymerization of both cellulose and lignin, which were often up-regulated since initial growth. These oxidative enzymes included lytic polysaccharide monooxygenases (LPMOs) acting on crystalline polysaccharides, cellobiose dehydrogenase involved in LPMO activation, and ligninolytic peroxidases (mainly manganese-oxidizing peroxidases), together with highly abundant H2O2-producing AAOs. Interestingly, some of the most relevant enzymes acting on polysaccharides were appended to a cellulose-binding module. This is potentially related to the non-woody habitat of P. eryngii (in contrast to the wood habitat of many basidiomycetes). Additionally, insights into the intracellular catabolism of aromatic compounds, which is a neglected area of study in lignin degradation by basidiomycetes, were also provided. The multiomic approach reveals that although non-woody decay does not result in dramatic modifications, as revealed by detailed 2D-NMR and other analyses, it implies activation of the complete set of hydrolytic and oxidative enzymes characterizing lignocellulose-decaying basidiomycetes. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This research was funded by the GENOBIOREF (BIO2017-86559-R) project of the Spanish Ministry of Science and Innovation (co-financed by FEDER funds) to F.J.R.-D. and S.C.; and by the Consejo Superior de Investigaciones CientÍficas (PIE-202120E019 to A.T.M. and PIE-201620E081 to A.G.). The work conducted by the JGI, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. DOE under contract DE-AC02-05CH11231. Part of this material is also based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research (BER) under the Early Career Award Program to D.S. This work was authored in part by the National Renewable Energy Laboratory, operated by Alliance for Sustainable Energy, LLC, for the U.S. Department of Energy (DOE) under Contract No. DE-AC36-08GO28308. The views expressed in the article do not necessarily represent the views of the DOE or the U.S. Government. The U.S. Government retains and the publisher, by accepting the article for publication |
| Databáze: | OpenAIRE |
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