Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization
| Autor: | B A Wentz, J Lane, M A Moore, Atin R. Datta |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Colony Count Microbial Biology medicine.disease_cause Sensitivity and Specificity Applied Microbiology and Biotechnology Horseradish peroxidase Microbiology chemistry.chemical_compound Nucleic acid thermodynamics Plasmid Listeria monocytogenes Genes Synthetic medicine Ecology Hybridization probe Nucleic Acid Hybridization Membranes Artificial biology.organism_classification Molecular biology chemistry Food Microbiology Nucleic Acid Renaturation Listeria biology.protein DNA Probes Molecular probe Phosphorus Radioisotopes DNA Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 59:144-149 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.59.1.144-149.1993 |
| Popis: | A plasmid containing the cloned listeriolysin gene of Listeria monocytogenes was used as a probe to identify Listeria strains by DNA colony hybridization. The probe DNA was labeled with horseradish peroxidase in the presence of glutaraldehyde. After the hybridization and wash procedures, the hybrid molecules were detected by luminescence, which resulted from the oxidation of luminol by a horseradish peroxidase-hydrogen peroxide-coupled reaction. Of the 150 Listeria strains and 16 non-Listeria strains examined, the probe hybridized only with L. monocytogenes. The technique was also used to enumerate L. monocytogenes in artificially contaminated foods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|