Effect of hyaluronic acid on paracrine signaling of osteoblasts from mesenchymal stromal cells: potential impact on bone regeneration
| Autor: | Eduardo Rolim Teixeira, Carina Lantmann Cabreira, Roberta Limeira Fulginiti, Rosemary Sadami Arai Shinkai, Patrícia Sesterheim |
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| Rok vydání: | 2020 |
| Předmět: |
Bone Regeneration
Cellular differentiation medicine.medical_treatment Fibroblast growth factor Extracellular matrix 03 medical and health sciences Paracrine signalling Mice 0302 clinical medicine Tissue engineering Osteogenesis Paracrine Communication medicine Animals Hyaluronic Acid Bone regeneration General Dentistry Cells Cultured Osteoblasts Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells 030206 dentistry Cell biology Cytokine Adipose Tissue 030220 oncology & carcinogenesis |
| Zdroj: | Clinical oral investigations. 25(7) |
| ISSN: | 1436-3771 |
| Popis: | This study evaluated hyaluronic acids (HA) with different molecular weights as potential matrices for tissue-engineered bone grafting and their possible influence on the paracrine mechanisms of adipose-derived mesenchymal stromal cells. Murine adipose mesenchymal stromal cells (mASCs) on the fourth passage were seeded in 96-well plates, osteoinduced for 27 days and exposed for 3 days to low (HA-LW) and high/low molecular weight (HA-HLW) at previously defined concentrations. Cytokines IGF-1, VEGF, FGF-2, and BMP-2 were evaluated by quantification in the supernatant. Greater expression of growth factors was observed in groups with HA-HLW compared to HA-LW. Results indicated that differentiated cells secreted fewer cytokines, namely VEGF, FGF, and BMP-2 than undifferentiated mASCs (p < 0.05). IGF-1 showed its greatest expression in the mASC HA-LW group (p < 0.05). The application of HA-HLW as cell matrix in tissue engineering did not compromise mASC paracrine effect. Also, the association of HA-HLW matrix and mASCs resulted in greater expression of osteogenic growth factors. Longer periods of cell differentiation seemed to negatively affect their capacity for local paracrine stimulation. The use of HA-HLW as matrix for undifferentiated ASCs can be positive for bone regeneration, favoring its application as cell matrix in bone grafting procedures. |
| Databáze: | OpenAIRE |
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