A sentinel protein assay for simultaneously quantifying cellular processes

Autor:	Christian von Mering, Stefanie Wanka, Alessio Maiolica, Paul J. Boersema, Marco Tognetti, Timon Wernas, Andre Melnik, Martin Soste, Paola Picotti, Rita Hrabakova
Přispěvatelé:	University of Zurich, Picotti, Paola
Rok vydání:	2014
Předmět:	Proteomics Saccharomyces cerevisiae Proteins Proteomics methods 1303 Biochemistry Systems biology Saccharomyces cerevisiae Computational biology Biochemistry Mass Spectrometry UFSP13-7 Evolution in Action: From Genomes to Ecosystems 1307 Cell Biology 03 medical and health sciences Protein Interaction Mapping 1312 Molecular Biology Humans Molecular Biology Bradford protein assay 030304 developmental biology 0303 health sciences biology Proteomic screening Cellular process Systems Biology 030302 biochemistry & molecular biology Computational Biology Reproducibility of Results Cell Biology Phosphoproteins biology.organism_classification Molecular biology 10124 Institute of Molecular Life Sciences 1305 Biotechnology 570 Life sciences Peptides Transcriptome Biotechnology
Zdroj:	NATURE METHODS Nature methods
Popis:	We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6b2803ef0c01ef50a76c977a280a5f7 https://www.zora.uzh.ch/id/eprint/100792/ Zobrazit plný text záznamu