Alpha-Glucosidase and Alpha-Amylase Inhibitory Activities of Novel Abietane Diterpenes from Salvia africana-lutea
| Autor: | Tesfaye Waryo, Ninon G.E.R. Etsassala, Emmanuel I. Iwuoha, Jeanine L. Marnewick, Jelili A. Badmus, Ahmed Hussein, Christopher N. Cupido |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Physiology Stereochemistry Clinical Biochemistry Salvia 01 natural sciences Biochemistry Article Carnosol Terpene 03 medical and health sciences chemistry.chemical_compound terpenoids Salvia africana-lutea alpha-glucosidase Molecular Biology Abietane chemistry.chemical_classification cape floristic region Lamiaceae biology 010405 organic chemistry lcsh:RM1-950 alpha-amylase Cell Biology biology.organism_classification Terpenoid 0104 chemical sciences lcsh:Therapeutics. Pharmacology 030104 developmental biology chemistry Alpha-glucosidase diabetes mellitus biology.protein Lactone |
| Zdroj: | Antioxidants Volume 8 Issue 10 Antioxidants, Vol 8, Iss 10, p 421 (2019) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox8100421 |
| Popis: | The re-investigation of a methanolic extract of Salvia africana-lutea collected from the Cape Floristic Region, South Africa (SA), afforded four new abietane diterpenes, namely 19-acetoxy-12-methoxycarnosic acid (1), 3&beta acetoxy-7&alpha methoxyrosmanol (2), 19-acetoxy-7&alpha methoxyrosmanol (3), 19-acetoxy-12-methoxy carnosol (4), and two known named clinopodiolides A (5), and B (6), in addition to four known triterpenes, oleanolic, and ursolic acids (7, 8), 11,12-dehydroursolic acid lactone (9) and &beta amyrin (10). The chemical structural elucidation of the isolated compounds was determined on the basis of one and two dimensional nuclear magnetic resonance (1D and 2D NMR), high-resolution mass spectrometry (HRMS), ultra violet (UV), fourier transform infrared (IR), in comparison with literature data. The in vitro bio-evaluation against alpha-glucosidase showed strong inhibitory activities of 8, 10, and 7, with the half inhibitory concentration (IC50) values of 11.3 ± 1.0, 17.1 ± 1.0 and 22.9 ± 2.0 µ g/mL, respectively, while 7 demonstrated the strongest in vitro alpha-amylase inhibitory activity among the tested compounds with IC50 of 12.5 ± 0.7 µ g/mL. Additionally, some of the compounds showed significant antioxidant capacities. In conclusion, the methanolic extract of S. africana-lutea is a rich source of terpenoids, especially abietane diterpenes, with strong antioxidant and anti-diabetic activities that can be helpful to modulate the redox status of the body and could therefore be an excellent candidate for the prevention of the development of diabetes, a disease where oxidase stress plays an important role. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|