Glucocorticoid regulation of mouse mammary tumor virus: identification of a short essential DNA region

Autor: H Diggelmann, E Buetti
Rok vydání: 1983
Předmět:
Gene Expression Regulation
Viral
animal structures
Transcription
Genetic
Base pair
viruses
Molecular Sequence Data
Response element
Biology
Transfection
Thymidine Kinase
General Biochemistry
Genetics and Molecular Biology
Mice
chemistry.chemical_compound
L Cells
Transcription (biology)
Animals
Simplexvirus
Glucocorticoids
Molecular Biology
Gene
Recombination
Genetic
Base Sequence
General Immunology and Microbiology
Animals Base Sequence *DNA
Viral Gene Expression Regulation
Viral/*drug effects Glucocorticoids/*pharmacology L Cells (Cell Line) Mammary Tumor Virus
Mouse/*genetics Mice Molecular Sequence Data Plasmids Recombination
Genetic Simplexvirus/enzymology/genetics Thymidine Kinase/genetics Transcription
Genetic Transfection
General Neuroscience
Mouse mammary tumor virus
biology.organism_classification
Molecular biology
Long terminal repeat
Mammary Tumor Virus
Mouse
chemistry
Thymidine kinase
DNA
Viral
DNA
Research Article
Plasmids
Zdroj: EMBO Journal, vol. 2, no. 8, pp. 1423-1429
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1983.tb01601.x
Popis: Transcription of mouse mammary tumor virus (MMTV) DNA is stimulated by steroid hormones. To determine the DNA sequences involved in this regulation, we constructed a plasmid containing the MMTV long terminal repeat (LTR) in front of the coding region of the herpes simplex thymidine kinase gene, from which the promoter had been removed. Portions of the LTR were removed by the nuclease Ba/31, and the deleted molecules were recloned and tested for transcriptional activity in transfections of Ltk-aprt- cells. Stably transfected cell clones were selected and hormone-dependent transcription from the MMTV promoter was studied by the S1 nuclease mapping method. The results show that DNA sequences between -105 and -204 base pairs upstream from the initiation site of viral transcription are required for glucocorticoid stimulation.
Databáze: OpenAIRE
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