Chemical-induced pH-mediated molecular switch
| Autor: | Dilani A. Jayawardhana, Xiyun Guan, Mrinal Kumar Sengupta, D. M. Milan Krishantha, Jyoti Gupta, Daniel W. Armstrong |
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| Rok vydání: | 2011 |
| Předmět: |
Molecular switch
Protein Folding beta-Cyclodextrins technology industry and agriculture Ionic Liquids Beta-Cyclodextrins Biosensing Techniques Hydrogen-Ion Concentration Combinatorial chemistry Article Analytical Chemistry Folding (chemistry) chemistry.chemical_compound Electrolytes Hemolysin Proteins Kinetics chemistry Covalent bond Ionic liquid Organic chemistry Hydroxymethyl Protein folding Biosensor Protein Unfolding |
| Zdroj: | Analytical chemistry. 83(20) |
| ISSN: | 1520-6882 |
| Popis: | The transmembrane protein α-hemolysin pore has been used to develop ultrasensitive biosensors, study biomolecular folding and unfolding, investigate covalent and non-covalent bonding interactions, and probe enzyme kinetics. Here, we report that by addition of ionic liquid tetrakis(hydroxymethyl)phosphonium chloride solution to the α-hemolysin pore, the α-hemolysin channel can be controlled open or closed by adjusting the pH of the solution. This approach can be employed to develop a novel molecular switch to regulate molecular transport, and should find potential applications as a ‘smart’ drug delivery method. |
| Databáze: | OpenAIRE |
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