Green fluorescent proteins with short half-lives as reporters in Dictyostelium discoideum
| Autor: | Shirin Friedel, Anne Detterbeck, Ursula Hamker, Heike Deichsel, Catharine Coyne, Harry K. MacWilliams |
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| Rok vydání: | 1999 |
| Předmět: |
Ribosomal Proteins
Green Fluorescent Proteins Gene Expression Dictyostelium discoideum Green fluorescent protein Ubiquitin Genes Reporter Ribosomal protein Gene expression Genetics Slime mold Animals Dictyostelium Promoter Regions Genetic Ubiquitins biology beta-Galactosidase biology.organism_classification Luminescent Proteins Microscopy Fluorescence Biochemistry biology.protein Indicators and Reagents Developmental Biology Deubiquitination |
| Zdroj: | Development Genes and Evolution. 209:63-68 |
| ISSN: | 1432-041X 0949-944X |
| Popis: | We describe two modifications of the popular reporter green fluorescent protein (GFP) which have short half-lives in our system, the cellular slime mould Dictyostelium discoideum. One of these bears an N-terminal ubiquitin; this GFP was originally planned to be a substrate of the "N-end-rule" pathway, but deubiquitination does not seem to occur, and a degradation by the UFD (ubiquitin-fusion-degradation pathway seems more probable. The protein half-life is about 3-5 h. The second construct has an N-terminus derived from the L11 ribosomal protein; it is transported to the nucleus and broken down much more rapidly than the ubiquitin fusion (protein half-life about 30 min). We show examples of the use of these reporters in the study of gene expression in Dictyostelium. |
| Databáze: | OpenAIRE |
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