Direct detection of brown adipose tissue thermogenesis in UCP1−/− mice by hyperpolarized 129Xe MR thermometry
| Autor: | Simone Degan, Andrew McCallister, Michael Antonacci, Christian T. McHugh, Michele Kelley, Rosa T. Branca |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Magnetic Resonance Spectroscopy Xenon animal structures Mr thermometry lcsh:Medicine Gene Expression Adipose tissue Thermometry White adipose tissue Article 030218 nuclear medicine & medical imaging Norepinephrine (medication) Mice Norepinephrine 03 medical and health sciences Magnetic resonance imaging Endocrinology 0302 clinical medicine Adipose Tissue Brown Internal medicine Brown adipose tissue medicine Animals lcsh:Science Uncoupling Protein 1 Mice Knockout Multidisciplinary Chemistry lcsh:R Thermogenesis Thermogenin 030104 developmental biology medicine.anatomical_structure Preclinical research Knockout mouse lcsh:Q Female Adrenergic alpha-Agonists medicine.drug |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-51483-4 |
| Popis: | Brown adipose tissue (BAT) is a type of fat specialized in non-shivering thermogenesis. While non-shivering thermogenesis is mediated primarily by uncoupling protein 1 (UCP1), the development of the UCP1 knockout mouse has enabled the study of possible UCP1-independent non-shivering thermogenic mechanisms, whose existence has been shown so far only indirectly in white adipose tissue and still continues to be a matter of debate in BAT. In this study, by using magnetic resonance thermometry with hyperpolarized xenon, we produce the first direct evidence of UCP1-independent BAT thermogenesis in knockout mice. We found that, following adrenergic stimulation, the BAT temperature of knockout mice increases more and faster than rectal temperature. While with this study we cannot exclude or separate the physiological effect of norepinephrine on core body temperature, the fast increase of iBAT temperature seems to suggest the existence of a possible UCP1-independent thermogenic mechanism responsible for this temperature increase. |
| Databáze: | OpenAIRE |
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