Application of DNA chip techniques for Yq microdeletion analysis in infertile males
| Autor: | Sook-Hwan Lee, Hyun Suk Joo, Jung-Eun Park, Seung Yong Hwang, Jae Hoon Hwang, Key Seung Cho, Suman Lee, Jong Man Kim |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Infertility DNA Mutational Analysis Clinical Biochemistry Obstructive azoospermia Biology Y chromosome Polymerase Chain Reaction Sensitivity and Specificity Biochemistry law.invention Male infertility Sequence-tagged site Predictive Value of Tests law medicine Humans Molecular Biology Infertility Male Sex Chromosome Aberrations Polymerase chain reaction Oligonucleotide Array Sequence Analysis Sequence Tagged Sites Electrophoresis Agar Gel Genetics Chromosomes Human Y Seminal Plasma Proteins medicine.disease genomic DNA Genetic Loci Molecular Medicine Female Chromosome Deletion DNA microarray |
| Zdroj: | Experimental & Molecular Medicine. 36:179-184 |
| ISSN: | 2092-6413 |
| Popis: | Our aim was to apply DNA chip technology as a diagnostic tool in infertility research and clinics. Six loci, including a sex-determining region on the Y chromosome and five sequence-tagged sites in azoospermia-factor regions were investigated in infertile male patients. Our method produced a sensitive signal, which showed the presence or absence of the STS regions on the Y chromosome. The results from 93 patients with non- obstructive azoospermia, oligoathenoteratozoospermia, or oligozoospermia were identical when analyzed with either the DNA chip technique or conventional PCR-gel electrophoresis. We have demonstrated its application in the molecular diagnosis of male infertility. This system provides an economic and high-throughput method for detecting the deletion of genomic DNA sequences of large groups of infertile patients, and a completely new approach to male infertility screening. The application of DNA chip technology to identify Yq deletions can also facilitate our understanding of male infertility. |
| Databáze: | OpenAIRE |
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