CCN1 enhances angiogenic potency of bone marrow transplantation in a rat model of hindlimb ischemia
Autor: | Yuan Liang, Xinghua Pan, Shu-guang Guo, Cun-ping Yin, Xing-li Zhou |
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Rok vydání: | 2014 |
Předmět: |
Angiogenesis
Muscle Fibers Skeletal Neovascularization Physiologic Apoptosis Bone Marrow Cells Stimulation Hindlimb Pharmacology Peripheral blood mononuclear cell Neovascularization Ischemia Cell Adhesion Human Umbilical Vein Endothelial Cells Genetics medicine Animals Humans Molecular Biology Bone Marrow Transplantation business.industry Genetic Therapy General Medicine Anatomy Recombinant Proteins Capillaries Rats Transplantation Disease Models Animal medicine.symptom business Perfusion Cysteine-Rich Protein 61 |
Zdroj: | Molecular Biology Reports. 41:5813-5818 |
ISSN: | 1573-4978 0301-4851 |
Popis: | Implantation of autologous bone marrow mononuclear cells (BM-MNCs) has been performed in ischemic tissues, for stimulation of angiogenesis, but the limited number of BM-MNCs in patients with hindlimb ischemia disease may offset their overall therapeutic efficacy. CCN1 is a novel and essential regulator during angiogenesis. We evaluated whether CCN1 and BM-MNC are capable of promoting angiogenesis in hindlimb ischemia. In this study, we created the rat model of hindlimb ischemia, and then the rats were randomly divided into four groups: CCN1 infusion plus BM-MNC transplantation (CCN1 + BM-MNCs group), CCN1 infusion plus PBS injection (CCN1 group), vehicle infusion plus BM-MNC transplantation (BM-MNCs group) and vehicle infusion plus PBS injection (control group). The combination of CCN1 and BM-MNC therapy could increase blood perfusion, capillary/muscle fiber ratio and tissue oxygenation in ischemic hindlimb. Moreover, CCN1 could not only inhibit the apoptosis of BM-MNCs, but also enhance the adhesiveness of BM-MNCs to HUVEC. Taken together, CCN1 enhanced angiogenesis of BM-MNC transplantation, and combining CCN1 with BM-MNC transplantation is a useful alternative for ischemic limbs. |
Databáze: | OpenAIRE |
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