Comparative analysis of the active sites of orthologous endolysins of the Escherichia lytic bacteriophages T5, RB43, and RB49
| Autor: | Svetlana I. Paskevich, Victor P. Kutyshenko, Dmitry A. Prokhorov, Galina V. Mikoulinskaia, Nikolai V. Molochkov, Vladimir N. Uversky, Alexander Y. Yegorov |
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| Rok vydání: | 2020 |
| Předmět: |
inorganic chemicals
Circular dichroism Stereochemistry Proton Magnetic Resonance Spectroscopy Lysin 02 engineering and technology Biochemistry Active center 03 medical and health sciences Viral Proteins Protein stability Structural Biology Escherichia Catalytic Domain Endopeptidases Escherichia coli Bacteriophages Amino Acid Sequence Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Circular Dichroism Active site General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology biology.organism_classification Enzyme Lytic cycle chemistry biology.protein bacteria Thermodynamics 0210 nano-technology |
| Zdroj: | International journal of biological macromolecules. 166 |
| ISSN: | 1879-0003 |
| Popis: | The methods of solution NMR, circular dichroism (CD), and differential scanning calorimetry (DSC) were used to study two zinc-containing L-alanyl-D-glutamate peptidases - endolysins of the pseudo T-even myoviruses RB43 and RB49 (EndoRB43 and EndoRB49, respectively), which are orthologous to the EndoT5, which is a zinc-containing L-alanyl-D-glutamate peptidase of the T5 siphovirus. The spatial conservation of the Zn2+-binding sites for the enzymes EndoT5, EndoRB43, and EndoRB49 was established, and the key role of Zn2+ ions in the stabilization of the spatial structures of these three peptidases was confirmed. We are showing here that the binding of the Zn2+ ion in the active center of EndoRB49 peptidase causes conformational rearrangements similar to those observed in the EndoT5 peptidase upon binding of Zn2+ and Ca2+ ions and lead to the formation of a catalytically active form of the enzyme. Therefore, the binding of the Zn2+ ion to the active site of EndoRB49 peptidase is a necessary and sufficient condition for functioning of this protein. |
| Databáze: | OpenAIRE |
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