Determination of 3-hydroxy pterocarpan, a novel osteogenic compound in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetics study
| Autor: | Lakshmi Manickavasagam, Ashutosh Raghuvanshi, Smriti Mishra, Amit Kumar, Girish Kumar Jain, Atul Goel, Sonal Gupta, Divya Singh |
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| Rok vydání: | 2010 |
| Předmět: |
Pterocarpans
Electrospray ionization Clinical Biochemistry Administration Oral Chemical Fractionation Sensitivity and Specificity Biochemistry Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Drug Stability Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Drug Discovery Animals Molecular Biology Pharmacology Detection limit Chromatography Elution Extraction (chemistry) Reproducibility of Results Estrogens General Medicine Isoflavones Rats Triple quadrupole mass spectrometer chemistry Linear Models Female Diethyl ether Quantitative analysis (chemistry) Chromatography Liquid |
| Zdroj: | Biomedical Chromatography. 25:843-850 |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.1527 |
| Popis: | A rapid, sensitive and selective LC-MS/MS method for the quantitative analysis of 3-hydroxy pterocarpan (S006-1709) in female rat plasma has been developed and validated. A Discovery RP18 column was used for the chromatographic elution using acetonitrile and 0.1% acetic acid in water as mobile phase (80:20 v/v) at the flow rate of 0.5 mL/min. MS/MS analysis was performed using a triple quadrupole mass spectrometer with electrospray ionization in negative ion mode using biochanin as an internal standard (IS). Extraction of S006-1709 and IS from rat plasma was done by liquid–liquid extraction method using diethyl ether. The LC-MS/MS method was sensitive with 1.95 ng/mL as the limit of detection and 3.9 ng/mL as the lower limit of quantification. The method was linear in the concentration range of 3.9–1000 ng/mL. The percentage bias for intraday and interday accuracy was not greater than 4.2 and the %RSD for intraday and interday precision was not greater than 13.2. The recoveries of S006-1709 and IS were 73.9–79.3 and 85.7%, respectively. S006-1709 was found to be stable in various stability studies. The validated LC-MS/MS method was successfully applied for the oral pharmacokinetics study of S006-1709 at 10 mg/kg in female Sprague–Dawley rats. Copyright © 2010 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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