Physiopathological responses of sole (Solea senegalensis) subjected to bacterial infection and handling stress after probiotic treatment with autochthonous bacteria

Autor: S. Batista, Luisa M.P. Valente, S. Cunha, José Gonçalves, A. Domingues, Ana M. P. Gomes, Benjamín Costas, Maria João Peixoto, Rodrigo O. A. Ozório
Přispěvatelé: Veritati - Repositório Institucional da Universidade Católica Portuguesa, CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Repositório Científico do Instituto Politécnico do Porto
Rok vydání: 2018
Předmět:
0301 basic medicine
Shewanella
fish disease
Solea senegalensis
ved/biology.organism_classification_rank.species
Glutathione reductase
peroxidase
law.invention
immunology
Fish Diseases
Probiotic
law
glutathione transferase
animal
lysozyme
innate immunity
probiotic agent
physiological stress
biology
Shewanella hafniensis
Enterococcus raffinosus
catalase
lipid peroxidation
04 agricultural and veterinary sciences
General Medicine
bacterium
Respiratory burst
Arthrobacter soli
Photobacterium damselae
priority journal
diet supplementation
Flatfishes
in vitro study
growth
animal experiment
Aquatic Science
Article
animal tissue
Microbiology
body weight
03 medical and health sciences
Photobacterium damselae subsp. piscicida
Immune system
nitric oxide
Stress
Physiological
bacterium isolation
transport stress
Animals
Environmental Chemistry
controlled study
Arthrobacter
intestine
glutathione reductase
growth
development and aging
complement alternative pathway
nonhuman
Photobacterium
ved/biology
animal model
Probiotics
Pseudomonas protegens
bacterial infection
biology.organism_classification
Diet
veterinary medicine
030104 developmental biology
Oxidative stress
Gram negative infection
placebo
040102 fisheries
flatfish
0401 agriculture
forestry
and fisheries
Gram-Negative Bacterial Infections
Enterococcus
Bacteria
Zdroj: Fish & Shellfish Immunology
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 1050-4648
Popis: This study aimed to evaluate the protective effects of four autochthonous bacteria isolated from juvenile sole (Solea senegalensis) intestine as dietary probiotic supplement against bacterial pathogen infection and handling/transport stressors. Growth performance and immune responses were evaluated after 85 days of feeding trial. Sole (IBW = 16.07 ± 0.11 g) were fed six experimental diets, a control diet (CTRL, without the dietary probiotic supplementation), and five diets supplemented with probiotic bacteria: PB1 (Shewanella hafniensis), PB2 (Enterococcus raffinosus), PB3 (Shewanella hafniensis + Arthrobacter soli), PB4 (Pseudomonas protegens + Arthrobacter soli) and PB5 (Shewanella hafniensis + Arthrobacter soli + Enterococcus raffinosus). All bacteria were selected based on their in vitro antimicrobial activity. After the growth trial, fish were submitted to a stress factor (transport) and then each dietary group was divided in two additional groups: non-infected (placebo) and infected with Photobacterium damselae subsp. piscicida. Immune and antioxidant responses were evaluated at day 10 post-infection. In infection trial A, fish were infected on the same day of transport, whereas in trial B fish were infected after a 7-day recovery from the transport stress. At the end of the feeding trial, fish fed with PB2 and PB4 showed lower final body weight when compared with the other dietary groups. Respiratory burst activity and nitric oxide production were not affected by probiotic supplementation. Fish fed with PB5 presented lower peroxidase activity compared to CTRL. Lysozyme and alternative complement pathway activity (ACH50) showed no significant differences between treatments. The innate immune responses were significantly affected after handling stress and bacterial infection. In trial A, the ACH50 levels of infected fish were significantly lower than the placebo groups. On the other hand, in trial B fish infected with Pdp demonstrated higher ACH50 levels when compared to placebos. Peroxidase levels were strongly modulated by bacterial infection and handling stress. In trials A and B, infection had a clear downgrade effect in peroxidase levels. Lipid peroxidation, catalase, glutathione S-transferase and glutathione reductase were altered by both bacterial infection and transport. Overall, dietary probiotic supplementation did not influence growth performance of sole. The immune and oxidative defenses of sole responded differently to infection depending on the probiotic and the synergy between pathogen infection and transport. © 2018 The current study was partially supported by the project PROBIOSOLEA (reference 13551), co-funded by Programa Operacional Regional do Norte (ON.2—O Novo Norte), under the Quadro de Referência Estratégico Nacional (QREN) , through the European Regional Development Fund . The current study was also supported by the Research Line INSEAFOOD of the project INNOVMAR-Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035 ), funded by the Northern Regional Operational Programme (NORTE 2020) through the ERDF. B. Costas was supported by FCT, Portugal ( IF/00197/2015 ).
Databáze: OpenAIRE
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