| Popis: |
The rate of oxygen evolution provides valuable information on the metabolic status and photosynthetic performance of a cell, and it can be quantified by means of a photosynthesis-irradiance (PI) curve. Up to now, the construction of PI curves of unicellular organisms based on oxygen evolution has been difficult and time consuming due to the lack of sensitive instruments. Here we describe the setup of a reproducible method for constructing PI curves based on oxygen evolution using small amounts of sample in the microalga Nannochloropsis gaditana, easily translatable to other algal species. |
