Mapping a multiplexed zoo of mRNA expression
| Autor: | Eric H. Davidson, Omar S. Akbari, Paul H. Patterson, Paul W. Sternberg, Mihoko Kato, Julius C. Barsi, Yuwei Li, Harry M. T. Choi, Benjamin E. Deverman, Barbara J. Wold, Sarkis K. Mazmanian, Scott E. Fraser, S. Melanie Lee, Bruce A. Hay, Carol Readhead, Rusty Lansford, Naeem Husain, Matt van de Rijn, Marianne E. Bronner, Jared R. Leadbetter, Anna C. T. Abelin, Tatjana Sauka-Spengler, Niles A. Pierce, Adam Z. Rosenthal, Dianne K. Newman, Colby R. Calvert, Ryan C. Hunter, David A. Prober, David Huss |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
In situ Embryo Nonmammalian animal structures Mrna expression ved/biology.organism_classification_rank.species Computational biology In situ hybridization Biology Multiplexing 03 medical and health sciences 0302 clinical medicine Techniques and Resources Animals Humans RNA Messenger Model organism Molecular Biology Zebrafish In Situ Hybridization Genetics High contrast ved/biology biology.organism_classification 030104 developmental biology Tissue sections Drosophila 030217 neurology & neurosurgery Developmental Biology |
| Popis: | In situ hybridization methods are used across the biological sciences to map mRNA expression within intact specimens. Multiplexed experiments, in which multiple target mRNAs are mapped in a single sample, are essential for studying regulatory interactions, but remain cumbersome in most model organisms. Programmable in situ amplifiers based on the mechanism of hybridization chain reaction (HCR) overcome this longstanding challenge by operating independently within a sample, enabling multiplexed experiments to be performed with an experimental timeline independent of the number of target mRNAs. To assist biologists working across a broad spectrum of organisms, we demonstrate multiplexed in situ HCR in diverse imaging settings: bacteria, whole-mount nematode larvae, whole-mount fruit fly embryos, whole-mount sea urchin embryos, whole-mount zebrafish larvae, whole-mount chicken embryos, whole-mount mouse embryos and formalin-fixed paraffin-embedded human tissue sections. In addition to straightforward multiplexing, in situ HCR enables deep sample penetration, high contrast and subcellular resolution, providing an incisive tool for the study of interlaced and overlapping expression patterns, with implications for research communities across the biological sciences. |
| Databáze: | OpenAIRE |
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