A new model for studying ischemia-reperfusion injury in hamster cheek pouch
| Autor: | Walter N. Durán, P. K. Dillon, Robert W. Hobson, H. Yasuhara |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Physiology Ischemia Muscle Smooth Vascular Microcirculation Venules Cheek pouch Cricetinae Physiology (medical) Cell Adhesion Leukocytes medicine Animals Mesocricetus business.industry Mouth Mucosa Blood flow Anatomy Cover slip Cheek medicine.disease Disease Models Animal medicine.anatomical_structure Reperfusion Injury Pouch Cardiology and Cardiovascular Medicine business Reperfusion injury Biomedical engineering |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 261:H1626-H1629 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1991.261.5.h1626 |
| Popis: | The cheek pouch was prepared as a single layer and attached to a two-piece Lucite chamber that was filled with bicarbonate buffer (pH 7.35, equilibrated with 95% N2-5% CO2). Two microcirculatory areas were circumscribed in the cheek pouch by equilateral triangular chambers. Each chamber was made of 90-microns thick Mylar sheet (2 mm wide, 18 mm long). A cover slip was used as the chamber top. Ischemia was induced by applying pressure to the cover slip. The experimental area was reperfused after 1 h of ischemia by releasing the pressure. The other area served as control. One hour of reperfusion after 1 h of ischemia caused a significant increase in the number of leukocytes adhering to postcapillary venules (PCVs) per 100 microns vessel length in the ischemic area relative to the values in the control area (7.8 +/- 2.5 vs. 3.7 +/- 1.3, respectively, for PCVs 10-19.9 microns diam; and 10.9 +/- 2.8 vs. 6.2 +/- 1.8 for PCVs 20-29.9 microns diam; P less than 0.01 for both comparisons). The results demonstrate the adequacy of the model to investigate leukocyte adhesion to endothelium in ischemia-reperfusion. Because blood flow is maintained in most of the pouch, our model should also be useful for identifying possible interactions between ischemic and nonischemic areas in the microcirculation. |
| Databáze: | OpenAIRE |
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