Biophysical characterization of a model antibody drug conjugate
| Autor: | Michael Storms, Tsutomu Arakawa, C.J. Okumura, Toshiaki Maruyama, Yasunori Kurosawa, Nasib Karl Maluf |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antibody-drug conjugate Circular dichroism Immunoconjugates Stereochemistry medicine.drug_class CHO Cells Monoclonal antibody Immunoglobulin G Biophysical Phenomena Analytical Ultracentrifugation 03 medical and health sciences Cricetulus Drug Delivery Systems medicine Animals Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Fluorescent Dyes biology Calorimetry Differential Scanning Chemistry Circular Dichroism Antibodies Monoclonal General Medicine Protein tertiary structure body regions 030104 developmental biology Spectrometry Fluorescence biology.protein Biophysics Rabbits Ultracentrifugation Fluorescein-5-isothiocyanate Conjugate |
| Zdroj: | Drug discoveriestherapeutics. 10(4) |
| ISSN: | 1881-7831 |
| Popis: | Antibody drug conjugates (ADC) are important next-generation biopharmaceuticals and thus require stringent structure characterization as is the case for monoclonal antibodies. We have tested several biophysical techniques, i.e., circular dichroism, analytical ultracentrifugation, differential scanning calorimetry and fluorescence spectroscopy, to characterize a fluorescein-labeled monoclonal antibody as a model ADC. These techniques indicated possible small structure and stability changes by the conjugation, while largely retaining the tertiary structure of the antibody, consistent with unaltered biological activities. Thus, the above biophysical techniques are effective at detecting changes in the structural properties of ADC. |
| Databáze: | OpenAIRE |
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